芦荟大黄素纳米脂质体介导的光动力疗法对人舌鳞状细胞癌细胞Tca-8113治疗效果的研究  

Therapeutic Effect of Aloe-emodin Nanometer Liposome-mediated Photodynamic Therapy on Human Tongue Squamous Cell Carcinoma Tca-8113 Cells

作　　者：邵明敬 王赫 董丽 张红超 刘旭[1] 孟培松[1] SHAO Ming-jing;WANG He;DONG Li;ZHANG Hong-chao;LIU Xu;MENG Pei-song(Department of Stomatology,the Fourth Medical College of Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学附属第四医院口腔科,黑龙江哈尔滨150001

出　　处：《口腔医学研究》2018年第9期948-951,共4页Journal of Oral Science Research

基　　金：哈尔滨市科学技术局项目(编号:2014RFXGJ095)

摘　　要：目的:探究芦荟大黄素纳米脂质体(aloe emodin nano-liposome,AE-L)介导的光动力疗法(photodynamic therapy,PDT)对人舌鳞状细胞癌(简称鳞癌)细胞Tca-8113的治疗效果。方法:逆相蒸发法制备AE-L;不同浓度AE-L(2.5、5.0、7.5、10.0、12.5μg/mL)作用Tca-8113细胞4h后,不同光照时间处理(0、10、20、40、80、160s;激发波长为405nm,功率密度为80 mW/cm2)Tca-8113,采用噻唑蓝(methyl thiazol tetrazolium,MTT)检测细胞存活率;AE-L(7.5μg/mL)作用细胞4h后,光照时间为80s(激发波长为405nm,功率密度为80mW/cm2)处理细胞,荧光显微镜定性分析PDT后活性氧(reactive oxygen species,ROS)含量。结果:AE-L浓度≤7.5μg/mL时细胞毒性较小,与对照组比较差异无统计学意义(P>0.05)。PDT组随着光照时间延长细胞存活率逐渐降低,光照时间80s时细胞存活率为76.6%,与对照组比较差异有统计学意义(P<0.001)。荧光染色结果显示,PDT治疗后细胞内ROS含量显著增加。结论:7.5μg/mL AE-L介导的PDT治疗对人舌鳞癌Tca-8113细胞具有显著的杀伤效果。Objective:To study the therapeutic effect of aloe-emodin nano-liposome-mediated photodynamic therapy(PDT)on human tongue squamous cell carcinoma Tca-8113 cells.Methods:Aloe-emodin nanosized liposomes(AE-L)were prepared by reverse phase evaporation method.Aloe-emodin absorption spectra were measured by ultraviolet spectrophotometer.The effects of different concentrations of AE-L(2.5,5.0,7.5,10.0,and 12.5μg/mL)Tca-8113 cells were treated with different light time(0,10,20,40,80,and 160 s;excitation wavelength at 405 nm;power density at 80 mW/cm 2).Cell viability was detected by MTT assay.After AE-L(7.5μg/mL)treated for 4 h,the irradiation time was 80 s,the ROS content was analyzed by fluorescence microscopy.Results:When the concentration of AE-L was less than 7.5μg/mL,there was not difference between PDT group and control group(P>0.05).With the increase of irradiation time,the cell survival rate decreased gradually.When the illumination time was 80 s,the cell survival rate was 76.6%,which is significant different from that of the control group(P<0.001).The results of fluorescent staining showed that the amount of ROS in the cells increased significantly after PDT treatment.Conclusion:The 7.5μg/mL AE-L-mediated PDT has a significant killing effect on human tongue squamous cell carcinoma Tca-8113 cells.

关 键 词：芦荟大黄素 脂质体 人舌鳞状细胞癌 光动力疗法 活性氧 

分 类 号：R739.86[医药卫生—肿瘤]