谷氨酰胺对高脂饮食诱导C57BL/6J小鼠肥胖和胰岛素抵抗的影响  

Effect of L-glutamine on obesity and insulin resistance in high-fat die T-induced C57BL/6J mice

作　　者：郭冰冰 蒋新液 裴晶晶 章恒 邱婷 卫雅蓉 GUO Bing-bing;JIANG Xin-ye;PEI Jing-jing;ZHANG Heng;QIU Ting;WEI Ya-rong(Wuxi Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University,Wuxi 214000,China)

机构地区：[1]南京医科大学附属无锡妇幼保健院儿保科,江苏无锡214000

出　　处：《中国病理生理杂志》2018年第9期1720-1723,共4页Chinese Journal of Pathophysiology

基　　金：南京医科大学科技发展基金资助项目(No.2015NJMU166);无锡市"科教强卫工程"医学青年人才项目(No.QNRC091)

摘　　要：目的:探讨谷氨酰胺(L-glutamine,Gln)对高脂饮食(high-fat diet,HFD)诱导小鼠肥胖和胰岛素抵抗的影响。方法:60只雄性C57BL/6J小鼠随机分为正常对照(normal control,NC)组、HFD组、HFD+丙氨酸(Lalanine,Ala)组和HFD+Gln组,每组15只。每周记录小鼠体重,给药16周后禁食不禁水12 h测定空腹血糖(fasting blood glucose,FBG),处死后剖腹取附睾脂肪垫并称重。采用酶联免疫法检测小鼠胰岛素(insulin,INS)、瘦素(leptin,LEP)、脂联素(adiponectin,APN)和胰高血糖素样肽1(glucagon-like peptide-1,GLP-1)的水平,并计算胰岛素抵抗指数(insulin resistance index,IRI)和胰岛素敏感指数(insulin sensitivity index,ISI)。结果:与NC组比较,HFD组小鼠体重和附睾脂肪垫重量明显升高,FBG、INS、IRI和LEP水平均明显升高,ISI和APN水平明显降低(P<0.05);与HFD组比较,HFD+Gln组小鼠体重明显下降,FBG和LEP水平明显降低,IRI明显减小(P<0.05)。4组小鼠血清的GLP-1水平差异无统计学显著性。结论:谷氨酰胺减轻高脂饮食诱导的肥胖小鼠体重和胰岛素抵抗。AIM:To explore the effect of L-glutamine(Gln)on obesity and insulin resistance in high-fat diet(HFD)-induced C57BL/6J mice.METHODS:Male C57BL/6J mice(n=60)were randomly divided into normal control(NC)group,HFD group,HFD+L-alanine(Ala)group and HFD+Gln group.Each group had 15 mice.The body weight of the mice was recorded weekly.Fasting blood glucose(FBG)of the mice was tested after 12-h fasting only with water-drinking at the end of the 16th week.The mice were sacrificed and epididymal fat pad was measured.The levels of insulin(INS),leptin(LEP),adiponectin(APN)and glucagon-like peptide-1(GLP-1)were measured by ELISA.The insulin resistance index(IRI)and insulin sensitivity index(ISI)were calculated.RESULTS:Compared with NC group,the body weight,epididymal fat pad weight,and the levels of FBG,INS,IRI and LEP increased significantly in HFD group(P<0.05),while the levels of ISI and APN decreased significantly(P<0.05).Compared with HFD group,the body weight,and the levels of FBG,IRI and LEP decreased significantly in HFD+Gln group(P<0.05),while the levels of ISI and APN increased significantly(P<0.05).No significant difference of serum GLP-1 levels the four groups was observed.CONCLUSION:L-glutamine reduces the body weight and attenuates the insulin resistance of HFD-induced mice.

关 键 词：谷氨酰胺 高脂饮食 肥胖 胰岛素抵抗 

分 类 号：R587[医药卫生—内分泌] R363.2[医药卫生—内科学]