肝细胞癌组织中Daxx的表达及临床意义  

Expressions of Daxx in hepatcellular carcinoma and its clinical significance

作　　者：王燕[1] 田薇[1] 刘颖[1] 靳钦[1] 张曙[1] WANG Yan;TIAN Wei;LIU Ying;JIN Qin;ZHANG Shu(Department of Pathology,Affiliated Hospital of Nantong University,Nantong,Jiangsu 226001,China)

机构地区：[1]南通大学附属医院病理科,江苏南通226001

出　　处：《重庆医学》2018年第25期3313-3318,共6页Chongqing medicine

摘　　要：目的探讨人肝细胞癌中死亡结构域相关蛋白(Daxx)的表达情况及其临床意义。方法组织芯片联合免疫组化法及Western blot检测Daxx在肝细胞癌中的表达情况;结合患者临床病理参数和随访资料进行统计学分析。结果 Daxx的表达主要定位于肝细胞癌的细胞质,正常肝组织的细胞核;Daxx的表达与AFP、肝炎史、肝硬化史、肿瘤分化程度、TNM分期、门静脉癌栓及Ki67表达相关(P<0.05);Kaplan-Meier生存曲线显示Daxx阴性组患者的总生存期(OS)与无进展生存期(PFS)更短;单因素生存分析显示Daxx表达程度、TNM分期、门静脉癌栓及Ki67表达程度与术后生存期相关;多因素COX比例风险回归模型分析进一步显示Daxx的表达程度、TNM分期及Ki67表达程度为患者独立的预后因素。结论 Daxx的异常表达可能与肝细胞癌的发生、发展及预后有着密切的关系。Objective To investigate the expression and clinical significance of Daxx in hepatocellular carcinoma.Methods The tissue microarray combined with immunohistochemistry and Western blot were used to detect the expression of Daxx in hepatocellular carcinoma.The statistical analysis was performed by combining with clinicopathological parameter and follow up data.Results The Daxx expression is mainly located in cytoplasm of hepatocellular carcinoma and neucleus in normal liver tissues.The Daxx expression was correlated with AFP,hepatitis history,cirrhosis history,degree of tumor differentiation,TNM staging,portal thrombosis and Ki67 expression.The Kaplan-Meier survival curves showed that the overall survival(OS)period and progress free survival(PFS)period in the Daxx lower expression group were shorter.The univariate survival analysis showed that the expression severity of Daxx,TNM staging,portal vein tumor thrombus and the Ki67 expression were associated with postoperative survival period.The multivariate COX proportional hazard regression model analysis further revealed that the Daxx expression severity,TNM staging and the Ki67 expression severity were the independent prognostic factors.Conclusion The abnorma1 expression of Daxx may have a close relationship with the occurrence,development and prognosis of hepatocellular carcinoma.

关 键 词：癌 肝细胞 DAXX 组织芯片 印迹法 蛋白质 

分 类 号：R735.7[医药卫生—肿瘤]