SSR分子标记分析云南引种油橄榄种质资源遗传多样性  

Genetic Diversity Analysis of Introduced Olive Germplasm Resources in Yunnan by SSR Markers

作　　者：耿树香 杨生超[1] 宁德鲁[2] 李勇杰 陈海云[2] GENG Shuxiang;YANG Shengchao;NING Delu;LI Yongjie;CHEN Haiyun(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Academy of Forestry,Kunming 650201,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南省林业科学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2018年第4期588-596,共9页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省重点新产品开发计划项目(2009BB006);中央财政推广项目(2017TG03)

摘　　要：【目的】对云南油橄榄出现不同品种之间的混淆、同种异名及同名异种进行准确鉴定。【方法】利用SSR荧光标记技术对云南永仁及丽江收集的82份油橄榄样品进行遗传多样性和分子鉴定。【结果】所筛选的20对引物共检测出181个等位变异,每对引物检测出3~14个等位变异,平均为9.05个。整个群体的观望杂合度为0.138 1~0.822 6之间,平均为0.489,期望杂合度为0.509 8~0.871 5之间,平均为0.721 6,PIC值在0.8以上的引物位点有3个,分别是DCA09、DCA18和GAPU71B。20个位点的扩增产物片断大约在53~233 bp之间。根据各品种的Nei’s遗传距离,运用UPGMA方法构建了所有品种的聚类图,结果 82份油橄榄参试样本被聚成了9个大组,选育品种与引种品种未被分开,但大多数具有相同品种名称的材料被分在同一大组中。【结论】云南油橄榄选育品种及引种品种均具有高水平遗传多样性。[Purpose]The confusion,homonyms and homonyms among different varieties of Olea europaea in Yunnan were accurately identified.[Method]Genetic diversity and molecular identification of 82 olive samples collected from Yunnan,Yongren and Lijiang were studied by SSR fluorescence labeling technique.[Results]181 alleles were detected by 20 pairs of primers,3–14 alleles were detected on each pair of primers,average of 9.05.Heterozygosity of the whole population ranged from 0.138 1 to 0.822 6,with an average of 0.489.The expected heterozygosity was between 0.509 8 and 0.871 5,with an average of 0.721 6,and 3 primers with PIC values above 0.8,they were DCA09,DCA18 and GAPU71B,respectively.The amplified product fragments of 20 loci are between 53 bp and 233 bp.According to Nei’s genetic distance among varieties,construct the dendrogram of all varieties by using UPGMA method,the results of 82 olive oil samples tested were clustered into 9 categories,breeding and varieties are not separate,but most have the same name of a variety of materials are in the same group.[Conclusion]Yunnan oil olive cultivars and varieties have high levels of genetic diversity.

关 键 词：油橄榄 SSR分子标记 遗传多样性 聚类分析 分子鉴定 

分 类 号：S565.702.4[农业科学—作物学]