铜锌复合胁迫对菜薹种子萌发、幼苗生长及子叶生理代谢的影响  

Effects of Combined Copper and Zinc Stress on the Seed Germination, Seedling Growth and Cotyledon Physiological Metabolism of Chinese Flowering Cabbage

作　　者：邹文桐[1] 曹智 ZOU Wentong;CAO Zhi(Key Laboratory of Measurement and Control System for Coastal Environment,Fuqing Branch of Fujian Normal University,Fuqing 350300,China;College of Ocean Science and Biochemistry Engineering,Fuqing Branch of Fujian Normal University,Fuqing 350300,China)

机构地区：[1]福建师范大学福清分校近海流域环境测控治理福建省高校重点实验室,福建福清350300 [2]福建师范大学福清分校海洋与生化工程学院,福建福清350300

出　　处：《云南农业大学学报（自然科学版）》2018年第4期632-639,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：近海流域环境测控治理福建省高校重点实验室2017年度开放课题(S1-KF1709);福建省教育厅B类项目(JB12263)

摘　　要：【目的】研究铜锌复合胁迫对菜薹种子萌发、幼苗生长及子叶生理代谢的影响,为探索菜薹生长发育的耐铜锌范围提供参考。【方法】以菜薹为试验材料,采用单因素随机设计,通过种子萌发试验研究铜锌复合胁迫处理(Cu^(2+)和Zn^(2+)的8个复合浓度分别为0 mmol/L+0 mmol/L、0.20 mmol/L+0.17 mmol/L、0.40 mmol/L+0.34 mmol/L、0.80 mmol/L+0.68 mmol/L、1.20 mmol/L+1.02 mmol/L、1.60 mmol/L+1.36 mmol/L、2.00 mmol/L+1.70 mmol/L和2.40 mmol/L+2.04 mmol/L)对菜薹种子萌发、幼苗生长等指标的影响。菜薹子叶中的SOD活性、CAT活性、可溶性糖、可溶性蛋白质和脯氨酸的含量均随铜锌复合胁迫的增加呈先增后减的趋势,均以处理T4 (0.80 mmol/L Cu^(2+)+0.68 mmol/L Zn^(2+))的最大,其值分别为30.76 U/(g·min)、327.81 U/(g·min)、11.24 nmol/g、15.46 mg/g、452.96μg/g;MDA含量、氧负离子产生速率(O2–)呈先减后增的趋势,以处理T4 (0.80 mmol/L Cu^(2+)+0.68 mmol/L Zn^(2+))的最小,其值分别为15.17 nmol/g和17.86 nmol/(g·min);POD活性和相对电导率呈上升趋势;发芽率、苗高、最长根长、单株鲜重和子叶光合色素含量均呈下降的趋势,均以处理T1(CK)的最大,其值分别为68.00%、1.64 cm、2.99 cm、18.32 mg、0.79 mg/g、0.20 mg/g、0.99 mg/g、0.19 mg/g。【结论】在受到铜锌复合胁迫时,菜薹种子萌发和幼苗生长受到抑制,但同时菜薹对较低的铜锌复合胁迫(0.80 mmol/L Cu^(2+)+0.68 mmol/L Zn^(2+))表现出一定的耐性。[Purpose]To study the effect of combined copper and zinc stress on the seed germination,seedling growth and cotyledon physiological metabolism of Chinese flowering cabbage to explore copper and zinc concentration scope of Chinese flowering cabbage’growth.[Method]By seed sprouting test,taking Chinese flowering cabbage as test material and combined copper and zinc as a variable,the effects of combined copper and zinc stress(eight treatments of combined Cu^2+and Zn^2+were 0 mmol/L+0 mmol/L,0.20 mmol/L+0.17 mmol/L,0.40 mmol/L+0.34 mmol/L,0.80 mmol/L+0.68 mmol/L,1.20 mmol/L+1.02 mmol/L,1.60 mmol/L+1.36 mmol/L,2.00 mmol/L+1.70 mmol/L and 2.40 mmol/L+2.04 mmol/L)on seed germination and seedling growth of Chinese flowering cabbage were studied.[Results]With the aggravation of combined copper and zinc stress,the SOD and CAT activities,contents of soluble sugar,soluble protein and proline of Chinese flowering cabbage cotyledon decreased after initial increase,which reached the peak value of 30.76 U/(g·min),327.81 U/(g·min),11.24 nmol/g,15.46 mg/g and 452.96μg/g with treatment T4.The MDA content and O2^– producing rate increased after initial decrease,which reached the bottom of 15.17 nmol/g and 17.86 nmol/(g·min)with treatment 4 and the POD activity and the relative conductivity increased gradually.The germination rate,seedling height,the longest root,fresh weight per plant and photosynthetic pigment contents of cotyledon decreased gradually,which reached the peak value of 68.00%,1.64 cm,2.99 cm,18.32 mg,0.79 mg/g,0.20 mg/g,0.99 mg/g,0.19 mg/g with treatment T1(CK).[Conclusion]Combined copper and zinc stress could stop the seed germination and seedling growth of Chinese flowering cabbage,and the tolerance of Chinese flowering cabbage to lower copper and zinc(0.80 mmol/L Cu^2++0.68 mmol/L Zn^2+)in adversity was observed.

关 键 词：铜锌复合污染 菜薹 种子萌发 幼苗生长 生理代谢 

分 类 号：S634.503.4[农业科学—蔬菜学]