禁食对大黄鱼幼鱼消化酶活性的影响研究  

Effect of Fast on Digestion Enzyme Activity of Juvenile Large Yellow Croaker(Larimichthys crocea)

作　　者：刘峰[1,2] 吕小康 刘阳阳[3] 楼宝 陈睿毅 LIU Feng;LV Xiao-Kang;LIU Yang-Yang;LOU Bao;CHEN Rui-Yi(The Key Lab of Mariculture and Enhancement of Zhejiang Province,Marine Fisheries Research Institute of Zhejiang,Zhoushan 316021,China;Marine and Fishery Institute of Zhejiang Ocean University,Zhoushan 316021,China;Marine Science and Technology College,Zhejiang Ocean University,Zhoushan 316022,China)

机构地区：[1]浙江省海洋水产研究所,浙江省海水增养殖重点实验室,浙江舟山316021 [2]浙江海洋大学海洋与渔业研究所,浙江舟山316021 [3]浙江海洋大学海洋科学与技术学院,浙江舟山316022

出　　处：《中国海洋大学学报（自然科学版）》2018年第A01期16-22,共7页Periodical of Ocean University of China

基　　金：浙江省重点研发计划项目(2016C02055-7);浙江省科技计划项目(2016F50043);舟山市科技计划项目(2016C31054);浙江海洋大学博士启动基金项目(22135010715)资助。

摘　　要：为了研究饥饿对大黄鱼(Larimichthys crocea)幼鱼体内消化酶活性的影响,以540尾40g左右的大黄鱼幼鱼为研究对象,分成6组(S0、S4、S8、S12、S16和S20),每组3个平行,分别禁食0、4、8、12、16和20d,禁食结束后每组取样9尾,每个平行3尾,测定并分析胃和肠道中消化酶(蛋白酶、脂肪酶和淀粉酶)活性。研究显示,禁食4d,胃和肠道中消化酶活性均出现较大幅度的降低(低于对照组28.32%~71.85%),随着禁食时间的延长,胃和肠道内3种消化酶活性均不同程度升高。在禁食4~20d时,胃内消化酶活性总体呈上升趋势,蛋白酶和脂肪酶活性在禁食16d时高于同期对照组,淀粉酶活性在禁食20d时高于同期对照组。肠道内消化酶活性呈先上升后下降趋势,其中,脂肪酶和淀粉酶活性在禁食8d时达到最高,蛋白酶活性在禁食12d时达到最高。禁食过程中,胃中蛋白酶活性始终高于肠道,但禁食过程中其变化幅度(-6.71%~63.72%)小于肠道(-64.79%~71.85%);脂肪酶和淀粉酶活性低于肠道,脂肪酶活性变化幅度(-62.88%~29.91%)小于肠道(-232.17%~46.28%),而淀粉酶活性的变化幅度(-81.71%~36.92%)大于肠道(2.35%~45.41%)。因此,与肠道相比,胃中蛋白酶和脂肪酶活性受到禁食的影响较大,淀粉酶活性受到的影响较小。此外,胃和肠道中淀粉酶活性均小于蛋白酶和脂肪酶。研究结果表明,脂肪酶和蛋白酶是大黄鱼幼鱼受到饥饿胁迫时参与代谢活动的主要酶类,而淀粉酶为从属酶类。本研究阐明了大黄鱼幼鱼饥饿过程中,体内消化酶活性的变化情况,为科学合理的投喂和大黄鱼的健康养殖提供了理论指导。In order to determine the effect of starvation on the digestive enzyme activity of Larimichthys crocea juvenile,540 juvenile individuals with body weight about 40 g were divided into six groups(S0,S4,S8,S12,S16,and S20,three replicates and 30 fish individuals each)and fasted for 0,4,8,12,16,and 20 d,respectively,0 as the control.After being fasted,nine fish individuals each group,3 each replicate were sampled for stomach and intestine digestive enzyme activity analyses.The analyzed included protease,lipase and amylase activities.The results showed that the activities of digestive enzymes in both stomach and intestine were influenced by fast significantly.The activities of three types of enzymes in both stomach and intestine all decreased sharply in the first 4 days of fast by 28.32%~71.85%,after that turned to rise to different extents.The digestive enzyme activities in stomach increased greatly from day 4 to day 20 while the activities of lipase and amylase in intestine rose from day 4 to day 8 and that of protease from day 4 to day 12,and then all the activities decreased.The activities of lipase and amylase in stomach were lower than those in intestine whereas the protease activity in stomach was higher than that in intestine,indicating that the food in stomach was digested limitedly by lipase and amylase.In addition,the ranges of protease and lipase activities in stomach(-6.71%~63.72%for protease,-62.88%~29.91%for lipase)were narrower than those in intestine(-64.79%~71.85%for protease,-232.17%~46.28%for lipase)whereas the range of amylase activity in stomach(-81.71%~36.92%)was higher than that in intestine(2.35%~45.41%).Thus,the effect of fast on the activities of protease and lipase in stomach were lower than that in intestine whereas the effect on amylase activity in stomach was higher than that in intestine.Furthermore,the amylase activity was lower than the protease and lipase activities in both stomach and intestine,suggesting that the lipase and protease were the main digestive enzymes involved in metab

关 键 词：大黄鱼 禁食 消化酶 分解代谢 

分 类 号：S968.1[农业科学—水产养殖]