Response of soil fungal community to long-term chromium contamination  

长期铬污染对土壤真菌群落的影响（英文）

作　　者：Jin HU De-long MENG Xue-duan LIU Yi-li LIANG Hua-qun YIN Hong-wei LIU 胡瑾;孟德龙;刘学端;梁伊丽;尹华群;刘宏伟(中南大学资源加工与生物工程学院,长沙410083;中南大学生物冶金教育部重点实验室,长沙410083)

机构地区：[1]School of Minerals Processing and Bioengineering,Central South University,Changsha 410083,China [2]Key Laboratory of Biometallurgy,Ministry of Education,Central South University,Changsha 410083,China

出　　处：《Transactions of Nonferrous Metals Society of China》2018年第9期1838-1846,共9页中国有色金属学报（英文版）

基　　金：Project(51504298)supported by the National Natural Science Foundation of China;Project(2016JJ3146)supported by the Natural Science Foundation of Hunan Province,China;Project(1053320171098)supported by the Fundamental Research Funds for the Central Universities of China;Project supported by the Postdoctoral Research Funding Plan in Central South University,China

摘　　要：To further study the fungal community in heavy metal contaminated ecosystems,soil samples were collected from an abandoned chromium(Cr)factory,and fungal community was analyzed by Illumina sequencing of Internal Transcribed Spacer(ITS)amplicons.The results showed that Cr contamination changed the composition and structure of soil fungal community,but didn’t change the diversity.Fungus showed various responses to Cr contamination.LEfSe analysis revealed that the biomarker changed a lot in the Cr-contaminated samples in comparison with that in the control samples.The changes in fungal community may be caused by the direct toxic effects on fungi by high concentration of Cr and the significant change in soil properties resulting from Cr contamination.Among all the Cr fractions,organic matter-bound Cr and exchangeable Cr showed significant effects on the fungal community and organic matter also showed a significant effect on soil fungal community.为进一步研究重金属污染生态系统中真菌群落的变化,以废弃铬盐厂污染的土壤为研究对象,通过二代测序ITS扩增子技术,研究真菌微生物群落。结果显示,铬污染虽然改变了真菌群落的组成和结构,但是对其多样性并没有显著的影响。不同种类的真菌对铬污染的响应不同。LEfSe分析结果显示,铬污染环境会导致真菌种类的变化,这是由于高浓度的铬污染能够对真菌细胞直接产生毒性,或者通过改变土壤性质,使真菌细胞对土壤中碳、氮等能源物质的利用能力下降。在所有铬的形态中,有机结合态和可交换态的铬对真菌群落的影响最显著;而土壤性质中,有机质对真菌群落的影响最为显著。

关 键 词：fungal community ALPHA-DIVERSITY BETA-DIVERSITY Cr contamination high-throughput sequencing 

分 类 号：X172[环境科学与工程—环境科学] X53