石墨烯/壳聚糖电化学传感器对色氨酸对映体的选择性识别  

Electrochemical Selective Recognition of Tryptophan Enantiomers Based on Graphene/Chitosan Composite Films

作　　者：张金萍 范景彪 黄艳[1] 米红宇[1] 耿建新[2] ZHANG Jinping;FAN Jingbiao;HUANG Yan;MI Hongyu;GENG Jianxin(Key Laboratory&Ministry of Oil&Gas Fine Chemicals,Ministry of Education Xinjiang Uyghur Autonomous Region,Xinjiang University,Urumqi 830046,Xinjiang,P.R.China;Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100190,P.R.China)

机构地区：[1]新疆大学化学化工学院石油天然气精细化工教育部&自治区重点实验室,新疆乌鲁木齐830046 [2]中国科学院理化技术研究所,北京100190

出　　处：《影像科学与光化学》2018年第5期398-407,共10页Imaging Science and Photochemistry

基　　金：国家自然科学基金(21462042;21262036;51773211)的支持

摘　　要：本文设计合成了一种石墨烯/壳聚糖复合物薄膜,并将其作为电化学电极应用于选择性检测具有旋光活性的色氨酸(Trp)对映体。采用X射线衍射、红外光谱、拉曼光谱、X射线光电子能谱、透射和扫描电子显微镜表征了石墨烯/壳聚糖复合物的结构。以石墨烯/壳聚糖复合物为电极,通过示差脉冲伏安法实现了Trp对映异构体的识别,结果表明L-Trp比D-Trp具有更高的电化学响应,并且峰电流密度和Trp的浓度在0.3×10-4~1.5×10-4 mol·L-1范围内有线性关系。该复合物电极具有厚度和面积可控、稳定性高等优点,可显著提高电化学检测中的电流信号,有望用于分子药物及临床医学领域中手性对映体的识别。In this research,graphene/chitosan composite films were designed and used as electrode for electrochemical enantioselective recognition of tryptophan(Trp)enantiomers.The structure of the graphene/chitosan composite films were characterized using X-ray diffraction,FT-IR spectroscopy,Raman spectroscopy,XPS spectroscopy,transmission and scanning electron microscopy.Trp enantiomers were recognized using graphene/chitosan composite electrode by a differential pulse voltammetry.Experimental data indicated that L-Trp exhibited stronger electrochemical response than D-Trp,and that a linear relationship between the current density of the oxidative peak and the concentrations of L-/D-Trp was established in a concentration span ranging from 0.3×10-4 mol·L^-1 to 1.5×10-4 mol·L^-1.The composite electrode had a number of advantages including controllable thickness and area,good stability,and enhanced electrochemical signals.Therefore,the graphene/chitosan composite electrodes are expected to be used for enantioselective recognition in areas such as molecular drugs and clinical medicine.

关 键 词：石墨烯 壳聚糖 示差脉冲伏安法 氨基酸 选择性识别 

分 类 号：Q517[生物学—生物化学] O657.1[理学—分析化学]