小枣黑疔病拮抗放线菌的筛选与发酵优化  

Screening and Opitimizing Fermentation Conditions of Actinomycetes spp. Antagonistic to Alternaria sp. Causing Black Spot of Jujube

作　　者：洪亮[1] 杨丽芬[1] 杨建[1] 郭建伟[1] 程加省[2] HONG Liang;YANG Li-fen;YANG Jian;GUO Jian-wei;CHENG Jia-sheng(Key Laboratory of Higher Quality and Efficient Cultivation and Security Control of Crops for Yunnan Province,Honghe College,Yunnan Mengzi 661100,China;Food Crops Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650205,China)

机构地区：[1]红河学院云南省农作物优质高效栽培与安全控制重点实验室,云南蒙自661100 [2]云南省农业科学院粮食作物研究所,云南昆明650205

出　　处：《西南农业学报》2018年第8期1634-1637,共4页Southwest China Journal of Agricultural Sciences

基　　金：云南省教育厅科研基金项目(2012C200);红河学院中青年学术骨干培养计划(2015GG0206);红河学院博士科研启动专项(XJ15B16);红河学院应用型研究项目(XJY15Y02);中山大学广东省热带亚热带植物资源重点实验室开放课题(PlantKF201503)

摘　　要：【目的】筛选蒙自小枣黑疔病链格孢拮抗放线菌,并优化发酵条件。【方法】以小枣黑疔病链格孢为指示菌,以肾茶26株、臭灵丹17株内生放线菌和大庆油田12株土壤放线菌作为供试菌,采用滤纸片法筛选高拮抗放线菌,进而通过单因子试验优化发酵条件。【结果】筛选到一株分离自肾茶根部、抑菌带宽达13.6 mm的拮抗菌株BEG-46,其最佳培养基组成和最佳培养条件为1 000 mL培养基中含葡萄糖2 g、酵母粉20 g,蛋白胨2 g,NaCl 4 g,CaCO_34 g,初始pH值为自然,发酵温度28℃,发酵周期为7 d;优化后BEG-46发酵液抑菌活性提高了118.3%。【结论】拮抗放线菌BEG-46的发酵液抑菌活性在优化条件下显著提升。【Objective】The aim was to screen out antagonistic Actinomycetes and optimize the fermentation conditions.【Method】Filter paper diffusing method was used to screen antagonistic Actinomycetes on Alternaria sp.causing black spot of Jujube from 26 strains endophyte-Actinomycetes of Lerodendranthus spicatus(Thunb.),17 strains of Laggera pterodonta(DC.)Benth.and 12 strains Actinomycetes spp.of Daqing Oil Field.Then single factor experimental method was exploited to optimize the fermentation conditions of the strain with the highest inhibition activities.【Result】One antagonistic Actinomycetes BEG-46 was selected from root of Clerodendranthus spicatus(Thunb.)C.Y.Wu,which exhibited the highest antagonistic activity(inhibition zone width up to 13.6 mm).The optimal fermentation conditions for antifungal substance production of BEG-46 were natural pH value,2 g/L glucose,20 g/L yeast,2 g/L peptone,4 g/L NaCl,4 g/L CaCO 3,liquid medium shaking for 7 days at 28℃.After optimized,the inhibition activity of fermentation broth of BEG-46 increased by 118.3%.【Conclusion】The inhibition activity of fermentation broth of BEG-46 was obviously increased in optimized fermentation conditions.

关 键 词：黑疔病 链格孢 拮抗放线菌 发酵条件 

分 类 号：S665[农业科学—果树学]