基于无性繁殖过程的蝙蝠蛾拟青霉循环发酵方法（英文）  

Recycling fermentation method of Paecilomyces hepiali based on asexual reproduction

作　　者：Sayed-Hassan-Raza Shah 陆震鸣 李硕硕 李华祥 罗志珊 张晓娟[2] 史劲松 许正宏[2] Sayed-Hassan-Raza Shah;LU Zhenming;LI Shuoshuo;LI Huaxiang;LUO Zhishan;ZHANG Xiaojuan;SHI Jinsong;XU Zhenghong(School of Pharmaceutical Science,Jiangnan University,Wuxi 214122,China;National Engineering Laboratory for Cereal Fermentation Technology,School of Biotechnology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学药学院,江苏无锡214122 [2]江南大学生物工程学院粮食发酵工艺与技术国家工程实验室,江苏无锡214122

出　　处：《中国酿造》2018年第9期34-38,共5页China Brewing

基　　金：国家自然科学基金(31401931);国家轻工技术与工程一流学科自主课题(LITE2018-11)。

摘　　要：蝙蝠蛾拟青霉(Paecilomyces hepiali)是分离自冬虫夏草(Ophiocordyceps sinensis)的一种无性型菌株,其发酵产物具有良好的生物活性。该研究利用蝙蝠蛾拟青霉在发酵培养基中液态发酵24 h后产生的无性孢子作为下一批次的发酵接种物,进行了20个批次的连续循环发酵,减少了发酵种子制备步骤。各批次发酵的生物量为(25.5~27.2 g/L),孢子数为(0.8×10~8~1.4×10~8spores/mL),腺苷产量为(49.5~62.3 mg/L),总三萜产量为(126.3~142.7 mg/L),发酵稳定性良好,没有统计学显著差异(P>0.05)。基于无性繁殖过程的蝙蝠蛾拟青霉循环发酵方法可以免去各批次菌种斜面培养和种子发酵制备、节约生产成本、提高生产效率。Paecilomyces hepiali was an asexual strain isolated from Ophiocordyceps sinensis,and its fermentation products had good bioactivity.The asexual spores produced by P.hepiali with liquid-state fermentation for 24 h were used as the inoculation of the next batch fermentation.A twenty-cycle reproduction-based repeated batch fermentation(RBF)process of P.hepiali was carried out,and the preparation process of fermented seed was reduced.The biomass,sporulation and bioactive components(adenosine and crude triterpenoids)of each fermentation cycle was determined,and biomass(25.5-27.2)g/L,spore numbers(0.8×108-1.4×108)spores/ml,adenosine production(49.5-62.3)mg/L,and crude triterpenoids production(126.3-142.7)mg/L showed good batch-to-batch fermentation stability throughout the fermentation process(P>0.05).The asexual reproduction-based RBF process of P.hepiali notably reduced cost and increased production efficiency since omitting out each batch of slant culture and seed fermentation each time.

关 键 词：蝙蝠蛾拟青霉 无性孢子 批次循环发酵 冬虫夏草 

分 类 号：Q939.97[生物学—微生物学]