RCS(rdy-/-,p-/-)大鼠虚中夹瘀证实验评价  

The Experimental Evaluation of the RCS(rdy-/-,p-/-)Rat with Blood Stasis and Deficiency Syndromes

作　　者：徐剑[1] 周亚莎 彭俊[1] 潘坤 李建超[1] 覃艮艳 彭清华[1] Jian Xu;Yasha Zhou;Jun Peng;Kun Pan;Jianchao Li;Genyan Qin;Qinghua Peng(Department of Ophthalmology,the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学第一附属医院眼科,长沙410007

出　　处：《中华眼视光学与视觉科学杂志》2018年第9期519-524,共6页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：湖南省教育厅创新平台开放基金项目(16K065);湖南中医药大学中医诊断学国家重点学科开放基金优先资助项目(2015ZYZD02);中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018);长沙市科技计划项目(kc1704005);中央财政支持地方高校建设项目。

摘　　要：目的:论证视网膜色素变性经典动物模型RCS(rdy-/-,p-/-)大鼠具有的“虚中夹瘀”的病理机制。方法:实验研究。将10只RCS(rdy+/+,p+/+)大鼠(雌雄各5只)设为空白组;RCS(rdy-/-,p-/-)大鼠共10只(雌雄各5只)设为模型组。观察2组大鼠的一般形态学及行为学特征,评价2组大鼠的生物学差异。ELISA法测定其虚证和瘀证指标,虚证指标包括视网膜匀浆组织中cAMP、cGMP含量和cAMP/cGMP值以及血浆中前列腺素(PG)、雌二醇(E2)含量;瘀证指标为血浆中P-selectin含量、血液流变学指标检测。组织切片HE染色观察2组大鼠视网膜结构。对2组数据行独立样本t检验。结果:模型组大鼠明显消瘦、神疲乏力、反应迟钝、行动缓慢、被毛蓬起无光泽、易受惊吓、食量小、大便略松软,其中雌性大鼠血浆中E2含量值明显低于空白组的雌性大鼠,差异有统计学意义(t=2.67,P=0.03);雄性大鼠血浆中PG含量值低于空白组的雄性大鼠,差异有统计学意义(t=4.12,P=0.003)。空白组大鼠视网膜组织匀浆cAMP、cGMP、cAMP/cGMP均高于模型组,差异有统计学意义(t=18.87、12.63、11.76,P<0.001)。当血流切变力分别为5/s、100/s、200/s时,模型组大鼠血黏度值高于空白组,差异有统计学意义(t=5.58、4.40、6.80,P<0.001)。模型组大鼠血浆黏度值、全血高切相对指数、全血低切相对指数、纤维蛋白原均高于空白组,差异有统计学意义(t=5.20,8.25,9.08,6.15,P<0.001)。模型组大鼠血浆中P-selectin含量明显高于空白组,差异有统计学意义(t=4.30,P=0.003)。模型组大鼠视网膜各层结构明显较空白组紊乱,视网膜厚度萎缩变薄,病变主要位于视网膜外层及视锥与视杆细胞。结论:视网膜色素变性经典模型RCS(rdy-/-,p-/-)大鼠具有形体消瘦,肾精亏虚且虚中加瘀等病理生理特点。Objective:To demonstrate that the RCS(rdy-/-,p-/-)rat model for retinitis pigmentosa has a blood stasis and deficiency pathogenesis.Methods:This was an experimental study.Ten RCS(rdy+/+,p+/+)rats were in the control group(5 males and 5 females);10 RCS(rdy-/-,p-/-)rats were in the model group(5 males and 5 females).The general morphological and behavioral characteristics of the two groups of rats were observed to evaluate the biological differences between them.ELISA was used to determine the indicators of blood stasis and deficiency.The indicators of deficiency included cAMP,cGMP and cAMP/cGMP levels in retinal homogenate tissue,and plasma PG and E2 levels.The indicators of blood stasis were the P-selectin level in plasma,and hemorheology indexes.Tissue sections with HE staining were used to observe the retinal structures of the rats in the two groups.Independent sample t tests were performed on both sets of data.Results:The rats in the model group manifested significant weight loss,fatigue,unresponsiveness,slow movements,volumized and dull back hair,vulnerability to fright,low food intake,and slightly loose stools.The plasma E2 content in female rats was significantly lower than that in the control group,and the difference was statistically significant(t=2.67,P=0.03).The plasma PG content in the male rats of the model group was lower than that of the control male rats,and the difference was statistically significant(t=4.12,P=0.003).The levels of cAMP,cGMP,and cAMP/cGMP in retinal tissue homogenates of the control group were higher than levels in the model group,and the difference was statistically significant(t=18.87,12.63,11.76,P<0.001).When the blood flow shear forces were 5/s,100/s,and 200/s,the blood viscosity of the model group was higher than that of the control group,and the difference was statistically significant(t=5.58,4.40,6.80,P<0.001).The plasma viscosity value,whole blood high incision relative index,whole blood low incision relative index,and fibrinogen in the model group were higher than those in

关 键 词：视网膜色素变性 RCS大鼠 虚证 瘀证 

分 类 号：R-332[医药卫生] R276.7