我国原发性青光眼患者外周血中含缬酪肽蛋白和α-胞衬蛋白含量的研究  

The levels of valosin-containing protein and alpha-fodrin in peripheral blood of patients with primary glaucoma in China

作　　者：吴德福 周利晓[1] 吕梁[1] 许冬 董敬民[2] WU De-Fu;ZHOU Li-Xiao;LV Liang;XU Dong;DONG Jing-Min(From the Department of Ophthalmology,the Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan Province,China;The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan Province,China)

机构地区：[1]郑州大学第五附属医院眼科,河南省郑州市450052 [2]郑州大学第一附属医院眼科,河南省郑州市450052

出　　处：《眼科新进展》2018年第10期968-971,共4页Recent Advances in Ophthalmology

基　　金：河南省医学科技攻关项目(编号:201503131)

摘　　要：目的检测不同类型青光眼患者与正常人外周血中含缬酪肽蛋白(valosin-containing protein,VCP)、α-胞衬蛋白(α-fodrin)的水平差异,评估各指标在青光眼诊断中的价值。方法收集2015年1月至2017年9月首诊于我院和郑州大学第一附属医院眼科的青光眼患者80例,其中原发性闭角型青光眼(primary angle-closure glaucoma,PACG)组40例,原发性开角型青光眼(primary open angle glaucoma,POAG)组40例;同期收集我院健康体检者40例作为对照组。采用ELISA法分别测定各组外周血中VCP和α-fodrin的含量,并作对比,同时对上述指标绘制受试者工作特征曲线,计算曲线下面积。采用多因素Logistic回归分析青光眼患者发病的危险因素。结果POAG组、PACG组和对照组VCP含量分别为(124.866±34.858)ng·L^(-1)、(94.143±28.970)ng·L^(-1)和(44.707±8.892)ng·L^(-1),α-fodrin含量分别为(0.531±0.306)μg·L^(-1)、(0.399±0.213)μg·L^(-1)、(0.304±0.214)μg·L^(-1),3组间两两相比,VCP、α-fodrin含量的差异均有统计学意义(均为P<0.05)。受试者工作特征曲线下面积分析结果显示,VCP为0.754(95%CI为0.646~0.862,P<0.001),α-fodrin为0.610(95%CI为0.486~0.733,P=0.091)。多因素Logistic回归分析提示,仅VCP是POAG患者发病的独立危险因素(OR=9.134,P<0.01)。结论POAG患者外周血中VCP和α-fodrin的含量高于PACG患者和正常人群,提示免疫因素可能在青光眼视神经损害机制中起重要作用,VCP是POAG发病的独立危险因素。Objective To detect the difference in valosin-containing protein(VCP)andα-fodrin contents in peripheral blood of patients with different types of glaucoma and normal people,and to evaluate the value of each index in the diagnosis of glaucoma.Methods Eighty patients with glaucoma for first diagnosis in our department and the Ophthalmology of the First Affiliated Hospital of Zhengzhou University from January 2015 to September 2017 were enrolled,including primary angle-closure glaucoma(PACG)group(40 patients)and primary open angle glaucoma(POAG)group(40 patients).Another 40 patients of healthy physical examination in our hospital were collected as control group during the same period.The contents of VCP andα-fodrin in peripheral blood of each group were determined by enzyme-linked immunosorbent assay(ELISA),followed by the comparison of the indicators.The receiver operating characteristic curve of the subjects were plotted.The area under the curve was calculated.Multivariate logistic regression was used to analyze the risk factors of glaucoma patients.Results The VCP content was(124.866±34.858)ng·L-1,(94.143±28.970)ng·L-1,(44.707±8.892)ng·L-1,whileα-fodrin concentration was(0.531±0.306)μg·L-1,(0.399±0.213)μg·L-1,(0.304±0.214)μg·L-1 in the POAG group,PACG group and control group,respectively.There were significant differences in the content of VCP andα-fodrin between the POAG group,PACG group and control group in pairwise comparison(all P<0.05).The area under the receiver operating characteristic curve analysis showed that the VCP was 0.754(95%CI 0.646-0.862,P<0.001)andα-fodrin was 0.61(95%CI 0.486-0.733,P=0.091).Multivariate logistic regression analysis indicated that only VCP was an independent risk factor for the pathogenesis of POAG(OR=9.134,P<0.01).Conclusion The contents of VCP andα-fodrin in peripheral blood of patients with POAG are higher than those of patients with PACG and normal controls.It suggests that immune factors may play an important role in the mechanism of glaucomatous optic

关 键 词：青光眼 免疫因素 含缬酪肽蛋白 Α-胞衬蛋白 

分 类 号：R775.1[医药卫生—眼科]