人结直肠癌中两种新分子生物标记物的筛选  

Two new molecular biomarkers in human colorectal cancer

作　　者：许特 王筠 赵宋礼 李延鹏 刘兴木[4] 钟姗 彭红瑜 屈军乐[2] 盛司潼 王晓梅 XU Te;WANG Yun;ZHAO Songli;LI Yanpeng;LIU Xingmu;ZHONG Shan;PENG Hongyu;QU Junle;SHENG Sitong;WANG Xiaomei(College of Life Sciences and Oceanography,Shenzhen University,Shenzhen 518060,Guangdong Province,P.R.China;College of Optoelectronic Engineering,Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,Shenzhen University,Shenzhen 518060,Guangdong Province,P.R.China;Pathology Department,People s Hospital of Sanshui District,Foshan 528100,Guangdong Province,P.R.China;Department of Abdominal Surgery,Shantou University Medical School Affiliated Second Hospital, Shantou 515065,Guangdong Province,P.R.China;Health Science Center,Shenzhen University,Shenzhen 518060,Guangdong Province,P.R.China)

机构地区：[1]深圳大学生命与海洋科学学院,广东深圳518060 [2]深圳大学光电工程学院,光电子器件与系统重点实验室,广东深圳518060 [3]佛山市三水区人民医院,广东佛山528100 [4]汕头大学医学院第二附属医院,广东汕头515065 [5]深圳大学医学部,广东深圳518060

出　　处：《深圳大学学报（理工版）》2018年第5期529-535,共7页Journal of Shenzhen University(Science and Engineering)

基　　金：国家重点研发计划资助项目(2016YFC0904601);深圳市科技计划资助项目(JCYJ20160520174247964)。

摘　　要：为挖掘结直肠癌早期病变的生物标记物,通过对高通量组织芯片上进行优化的多重免疫组织化学染色,观察比对了TATA序列结合蛋白相关因子1 (TATA-box binding protein associated factor 1 like,TAF1L)和stomatin家族蛋白1 (stomatin-like protein 1,STOML1)在结直肠癌组织和相匹配的癌旁正常组织之间的表达差异,检测了其表达水平与癌组织的不同临床分期、病理分级和tumor-lymph node-metastasis(TNM)分期的关系.结果显示,相比癌旁正常组织,TAF1L蛋白质在癌组织中呈高表达,差异具有统计学显著性意义(P <0. 000 1),并可随原位病灶的恶化显现明显的表达改变; STOML1蛋白质的表达水平在癌组织中差异无明显统计学意义(P> 0. 05).结果显示,TAF1L蛋白质的异常高表达与结直肠癌的发生发展可能存在直接的关联,有可能成为结直肠癌早期诊断、精准治疗和预后判定的新分子标记物.In order to find new molecular biomarkers for early diagnose of colorectal cancer(CRC),we investigated the potential role of TATA-binding protein factor associated 1 like(TAF1L)and stomatin-like protein 1(STOML1)in colorectal cancers and matched normal adjacent tissues by using tissue microarray with multiple immunohistochemical staining.Moreover,the relationship between TAF1L expressive level and deteriorated stages of CRC was also analyzed.The results showed that protein expression of TAF1L was significantly higher in CRC tissues than that in normal adjacent tissues(p<0.0001),and abnormal higher levels of TAF1L were significantly associated with the deterioration of CRC.In contrast,protein expression of STOML1 showed no statistical difference between colorectal cancer and normal adjacent tissue(p>0.05).The conclusion suggests that TAF1L overexpression is probably related to the occurrence and development of colorectal cancer,and may also be as a potential biomarker for early diagnosis,precision medicine and prognosis of colorectal cancer.

关 键 词：结直肠癌 组织芯片 多重免疫组织化学染色 TATA序列结合蛋白相关因子1 stomatin家族蛋白1 生物标志物 

分 类 号：R735.35[医药卫生—肿瘤] R363.14[医药卫生—临床医学]