大蒜素对模型小鼠的抗炎性痛作用及机制研究  

Study on the Mechanism of Anti-inflammatory Pain Effect of Allicin in Model Mice

作　　者：任亮[1] 任翔[2] 刘金宝[1] 朱吾元[1] 杜钢军[3] REN Liang;REN Xiang;LIU Jinbao;ZHU Wuyuan;DU Gangjun(Dept.of Pharmacology,Luohe Medical College,Henan Luohe 462000,China;Grade 2015 of Pharmacy Major,China Pharmaceutical University,Nanjing 211198,China;College of Pharmacy,Henan University,Henan Kaifeng 475004,China)

机构地区：[1]漯河医学高等专科学校药理教研室,河南漯河462000 [2]中国药科大学药学专业2015级,南京211198 [3]河南大学药学院,河南开封475004

出　　处：《中国药房》2018年第18期2479-2483,共5页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81472745);河南省科技攻关计划项目(No.豫科〔2013〕25号-132102310455)。

摘　　要：目的:研究大蒜素对模型小鼠的抗炎性痛作用及机制。方法:50只BALB/c小鼠随机分为模型组(等体积0.9%氯化钠溶液)、阿司匹林组(200 mg/kg)和大蒜素高、中、低剂量组(40、20、10 mg/kg),每组10只;均灌胃给药,每天1次,连续3 d。末次给药1 h后给予小鼠腹腔注射0.7%冰醋酸溶液(0.2 m L)建模,记录建模15 min内其扭体反应次数。分组与给药同上,末次给药1 h后给予小鼠左后足底皮下注射1.5%甲醛溶液(25μL)建模,记录建模后0~10 min(第Ⅰ时相)和10~60 min(第Ⅱ时相)时小鼠累计舔足时间。分组与给药同上,末次给药1 h后给予小鼠右耳廓正反两面均匀涂抹二甲苯(30μL)建模,建模2 h后测定小鼠耳廓肿胀度,以两耳质量差值为耳廓肿胀度,并计算耳廓肿胀抑制率。50只BALB/c小鼠随机分为空白对照组(等体积0.9%氯化钠溶液)、模型组(等体积0.9%氯化钠溶液)和大蒜素高、中、低剂量组(40、20、10 mg/kg),每组10只;均灌胃给药,每天1次,连续3 d。末次给药20 min后给予小鼠足趾皮下注射1%角叉菜胶0.9%氯化钠溶液(30μL)建模,分别于建模前及建模1、3、5 h后测定小鼠足趾容积,以建模前后小鼠足趾容积差值为足趾肿胀度;测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量以及总抗氧化能力(T-AOC)水平;采用Western blot法检测核转录因子κB(NF-κB)、肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)蛋白表达。结果:与模型组比较,阿司匹林组和大蒜素高、中剂量组小鼠扭体反应次数均显著减少;第Ⅰ时相时大蒜素高、中剂量组和第Ⅱ时相时阿司匹林组及大蒜素高、中剂量组小鼠累计舔足时间均显著缩短;阿司匹林组和大蒜素高剂量组小鼠耳廓肿胀度均显著降低;建模1、3 h后大蒜素高、中剂量组和建模5 h后大蒜素高剂量组小鼠足趾肿胀度均显著降低,差异均有统计学意义(P<0.05或P<0.01)。与空白对照组OBJECTIVE:To study the mechanism of anti-inflammatory pain effect of allicin in model mice.METHODS:Totally 50 BALB/c mice were randomly divided into model group(constant volume of 0.9%sodium chloride solution),aspirin group(200 mg/kg),allicin high-dose,medium-dose and low-dose groups(40,20,10 mg/kg),with 10 mice in each group.They were given relevant medicine intragastrically,once a day,for consecutive 3 d.One hour after last medication,they were given intraperitoneal injection of 0.7%glacial acetic acid solution(0.2 mL);the times of writhing response were recorded with in 15 min after modeling.Grouping and administration were same as above;1 h after last medication,mice were given subcutaneous injection of 1.5%formaldehyde solution(25μL)via left back toe;accumulative licking time of mice were recorded 0-10 min(time phaseⅠ)and 10-60 min(time phaseⅡ)after modeling.Grouping and administration were same as above;1 h after last medication,mice were given xylene(30μL)on both sides of right ear;the degree of auricular swelling of mice was determined 2 h after modeling.The mass difference of two ears was regarded as the degree of auricular swelling and then the inhibitory rate of swelling was calculated in mice.Totally 50 BALB/c mice were randomly divided into blank control group(constant volume of 0.9%sodium chloride solution),model group(constant volume of 0.9%sodium chloride solution)and allicin high-dose,medium-dose and low-dose groups(40,20,10 mg/kg),with 10 mice in each group.They were given relevant medicine intragastrically,once a day,for consecutive 3 d.Twenty minutes after last medication,mice were given subcutaneous injection of 1%carrageenan 0.9%sodium chloride solution(30μL)via toe.Toe volume of mice was determined before modeling and 1,3,5 h after modeling.The difference of toe volume in mice was regarded as the degree of toe swelling.The activities of SOD and GSH-Px,contents of MDA,and level of T-AOC were determined.The expression levels of NF-κB,TNF-αand IL-1βprotein were detected by Western bl

关 键 词：大蒜素 炎性痛 氧化应激 核转录因子ΚB 肿瘤坏死因子α 白细胞介素1Β 

分 类 号：R285[医药卫生—中药学]