中药白芍亲缘性分析及分子鉴定标记的筛选  

Analysis the affinities of Radix paeoniae Alba and screening of molecular identification markers

作　　者：李敏[1] 洪露 茅学群 LI Min;HONG Lu;MAO Xuequn(College of Phamacy,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江工业大学药学院,浙江杭州310014

出　　处：《浙江工业大学学报》2018年第5期581-584,共4页Journal of Zhejiang University of Technology

摘　　要：提取4种白芍的DNA基因组,筛选26条随机引物对浙白芍、亳白芍、山白芍和川白芍进行PCR扩增,共扩增出385条带,多态百分率为89%.聚类分析得到山白芍与亳白芍遗传相似性系数最高(为0.723 7),而与浙白芍的遗传相似性系数最低(为0.520 2).其中引物S17,S7,S61,S98筛选到浙江白芍、安徽白芍和山东白芍的分子鉴定标记.实验筛选获得的分子鉴定标记经克隆和测序后,可为白芍的特异性PCR鉴定的引物设计打下基础,也为其他中药材品种的分子水平的鉴定研究提供参考.Genomic DNA of four kinds of Radix paeoniae Alba was extracted.26 random primers were used to do RAPD screening,including the template of Radix paeoniae Alba genomic DNA from Zhejiang,Anhui,Shandong and Sichuan.The results showed that 385 bands were amplified,the polymorphic ratio was 89%.The clustering analysis showed that the genetic similar coefficient of Radix paeoniae Alba between Shandong and Anhui was 0.7237,which was the highest,while the genetic similarity coefficient of Radix paeoniae Alba between Shandong and Zhejiang was 0.5202,the lowest.Moreover,by primer S17,S7,S61 and S98,molecular identification of Radix paeoniae Alba from Zhejiang,Anhui and Shandong were screened.If those molecular markers cloned and sequenced in the next step,the specific PCR identification primers of Radix paeoniae Alba could be designed,which would construct the identification method of Radix paeoniae Alba.The data provides a good reference for the molecular identification of other Chinese traditional medicines.

关 键 词：白芍 多态百分率 中药分子鉴定标记 

分 类 号：R931.5[医药卫生—生药学]