白藜芦醇对胶质瘤U87细胞上皮-间质转化的抑制作用  

Inhibitory effect of resveratrol on epithelial to mesenchymal transition of glioma U87 cells

作　　者：赵丽艳[1] 宋扬[2] 陈勇[2] 吕晓艳[1] 贾茗博 李蕴潜[2] ZHAO Liyan;SONG Yang;CHEN Yong;LYU Xiaoyan;JIA Mingbo;LI Yunqian(Department of Medical Laboratory,Second Hospital,Jilin University,Changchun 130041,China;Department of Neurosurgery,First Hospital,Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学第二医院检验科,吉林长春130041 [2]吉林大学第一医院神经外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2018年第5期943-948,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目资助课题(20150414024GH)

摘　　要：目的:探讨白藜芦醇(Res)对胶质瘤U87细胞上皮-间质转化(EMT)、迁移和侵袭能力的调控作用,阐明Res对胶质瘤细胞EMT的抑制作用。方法:将胶质瘤U87细胞分为对照组(不做处理)、EMT组(TGF-β1诱导EMT)和Res处理组(Res处理EMT)。应用Western blotting法检测各组细胞中间质标志物(N-钙黏蛋白、β-连环蛋白和波形蛋白)、诱导EMT的转录因子(Snail、Slug和Twist1)以及基质金属蛋白酶(MMP)-2和MMP-9的蛋白表达水平。划痕愈合实验检测细胞迁移能力,Transwell侵袭实验检测细胞侵袭能力。结果:与对照组比较,EMT组胶质瘤U87细胞的间质标志物(N-钙黏蛋白、β-连环蛋白和波形蛋白)、诱导EMT的转录因子(Snail、Slug和Twist1)和MMP-2、MMP-9蛋白表达水平均明显升高(P<0.05);与EMT组比较,Res处理组上述蛋白表达水平明显下调(P<0.05和P<0.01);40μmol·L^(-1) Res处理组效果较20μmol·L^(-1) Res处理组更明显(P<0.05)。EMT组细胞迁移率和侵袭率明显高于对照组(P<0.01),而Res处理组胶质瘤细胞迁移率和侵袭率明显低于EMT组(P<0.01)。结论:Res能抑制胶质瘤U87细胞EMT,降低细胞的迁移和侵袭能力。Objective:To investigate the regulatory effects of resveratrol(Res)on the epithelial to mesenchymal transition(EMT),migration and invasion abilities of the glioma U87 cells,and to clarify the inhibitory effect of Res on EMT of the cells.Methods:The glioma U87 cells were divided into control group(no treatment),EMT group(EMT was induced with TGF-β1)and Res treatment group(EMT was treated with Res).The expression levels of mesenchymal markers(N-cadherin,β-catenin,Vimentin),EMT-inducing transcription factors(Snail,Slug,Twist1),matrix metalloproteinase(MMP)-2 and MMP-9 in the glioma U87 cells were measured by Western blotting method.Scratch-healing assay and Transwell assay were performed to examine the cell migration and invasion abilities.Results:Compared with control group,the protein expression levels of mesenchymal markers(N-cadherin,β-catenin,Vimentin),EMT-inducing transcription factors(Snail,Slug,Twist1),and MMP-2 and MMP-9 in EMT group were significantly increased(P<0.05).Compared with EMT group,the expression levels of above proteins in Res treatment group were markedly down-regulated(P<0.05 or P<0.01);the effect in 40μmol·L-1 Res treatment group was more obvious than that in 20μmol·L-1 Res treatment group.The migration and invasion rates of the U87 cells in EMT group were obviously elevated compared with control group(P<0.01),and the migration and invasion rates of the glioma U87 cells in Res treatment group were significantly lower than those in EMT group(P<0.01).Conclusion:Res can inhibit EMT of the glioma U87 cells and reduce the migration and invasion abilities of the cells.

关 键 词：白藜芦醇 胶质瘤 U87细胞 上皮-间质转化 细胞迁移 细胞侵袭 

分 类 号：R739.41[医药卫生—肿瘤]