纳米载银室温固化型PMMA材料对小鼠肝组织和肝细胞DNA损伤的影响  

Effects of room curing polymethyl methacrylate with nano-silver on liver tissue and DNA damage of hepatic cells in mice

作　　者：史金先 张赐童 刘璐 黄天意 闫通通 孙世群[1] 王晓容[1] SHI Jinxian;ZHANG Citong;LIU Lu;HUANG Tianyi;YAN Tongtong;SUN Shiqun;WANG Xiaorong(Department of Prosthodontics,Stomatology Hospital,Jilin University,Changchun 130021,China;Key Laboratory of Tooth Development and Bone Remodeling and Regeneration,Jilin Province, Changchun 130021, China)

机构地区：[1]吉林大学口腔医院修复科,吉林长春130021 [2]吉林省牙发育及颌骨重塑与再生重点实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2018年第5期962-967,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省发展改革委员会产业创新专项资金项目资助课题(2016C045-2);吉林省卫计委卫生技术创新项目资助课题(2017J070)

摘　　要：目的:探讨纳米载银室温固化型聚甲基丙烯酸甲酯(PMMA)材料对雄性小鼠肝脏组织和肝细胞DNA损伤的影响,评估该纳米复合材料的肝脏毒性。方法:40只6~8周龄雄性昆明小鼠随机分为纳米载银室温固化型PMMA组(72h浸提液、1/2 72h浸提液、1/4 72h浸提液)、室温固化型PMMA组(72h浸提液、1/2 72h浸提液和1/4 72h浸提液)、阴性对照组和阳性对照组(n=5)。以经口灌胃的方式,在实验开始、24h和45h给予除阳性对照剂之外的受试物,阳性对照剂甲磺酸乙酯(EMS)仅在24和45h经口灌胃。阴性对照组小鼠灌胃给予等量生理盐水。最后一次给药3h后摘小鼠眼球取血,行肝脏生化指标检测;处死小鼠后立即完整取出肝脏,计算脏器系数;体内彗星实验(CA)检测尾部DNA百分比(%tail DNA)、尾长(TL)和Olive尾矩(OTM),采用彗星图像分析软件进行图像分析。结果:与阴性对照组比较,各实验组小鼠肝脏系数、丙氨酸氨基转移酶(ALT)和门冬氨酸氨基转移酶(AST)水平比较差异均无统计学意义(P>0.05);与阴性对照组比较,纳米载银室温固化型PMMA组和普通室温固化型PMMA组不同浓度浸提液组尾部%tail DNA、TL和OTM比较差异均无统计学意义(P>0.05)。阳性对照组小鼠%tail DNA、TL和OTM明显高于阴性对照组(t=-40.911,P<0.05;t=-16.620,P<0.05;t=32.943,P<0.05)。相同浓度下纳米载银室温固化型PMMA组%tail DNA、TL和OTM与室温固化型PMMA组比较差异均无统计学意义(P>0.05)。纳米载银室温固化型PMMA组不同浓度浸提液%tail DNA、TL和OTM比较差异无统计学意义(P>0.05)。结论:纳米载银室温固化型PMMA材料未对小鼠肝脏及其功能产生不良影响,彗星实验结果提示其不具有遗传毒性,表明该复合材料具有良好的生物相容性。Objective:To study the effects of room curing polymethyl methacrylate(PMMA)with nano-silver on the liver tissue and DNA damage of hepatic cells in the mice,and to evaluate the hepatotoxicity of the nanocomposites.Methods:A total of 40 male Kunming mice aged 6-8 weeks were divided into room curing PMMA with nano-silver groups(PMMA-NM groups,72 h extract liquid,1/2 72 h extract liquid,1/4 72 h extract liquid),room curing PMMA groups(PMMA groups,72 h extract liquid,1/2 72 h extract liquid,1/4 72 h extract liquid),negative control group,and positive control group(n=5).All mice were treated by gavage with test compounds at the beginning of experiment and 24 h and 45 h after experiment except the positive control agent,the mice in positive control group were treated with ethylmethylsulfone at 24 and 45 h after experiment,and the mice in negative control group were administrated with the same volume of normal saline by gavage.The eyeball blood of mice was collected for biochemistry analysis of liver 3 h after the final administration.The liver tissue was completely removed immediately after the mice were sacrificed and the organ coefficient was calculated.The percentage of tail DNA(%tail DNA),tail length(TL)and Olive tail moment(OTM)were detected by the in vivo comet assay,and the images were analyzed with analysis software of comet assay.Results:Compared with negative control group,the liver coefficient,the alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels in liver tissue of the mice in various experimental groups had no significant differences(P>0.05);the%tail DNA,TL and OTM in PMMA-NM groups and PMMA groups had no significant differeces(P>0.05).The%tail DNA,TL and OTM of the mice in positive control group were higher than those in negative control group(t=-40.911,P<0.05;t=-16.620,P<0.05;t=32.943,P<0.05).There were no significant differences of the%tail DNA,TL and OTM of the mice between PMMA-NM groups and PMMA groups at the same concentration(P>0.05).There were no significant differences of the

关 键 词：纳米载银无机抗菌剂 聚甲基丙烯酸甲酯 彗星实验 DNA损伤 遗传毒性 

分 类 号：R783.1[医药卫生—口腔医学]