水铁矿对玉米生长及其抗氧化系统的影响  

Effect of ferrihydrite on maize growth and antioxidant system

作　　者：古芸 郑赛 曲峰龙 王韬略 余光辉[1] 冉炜[1] 沈其荣[1] GU Yun;ZHENG Sai;QU Fenglong;WANG Taolue;YU Guanghui;RAN Wei;SHEN Qirong(College of Resources and Environmental Sciences/Jiangsu Provincial Key Laboratory for Solid Organic Waste Utilization,Nanjing 210095,China)

机构地区：[1]南京农业大学资源与环境科学学院/江苏省固体有机废弃物资源化利用高技术研究重点实验室,江苏南京210095

出　　处：《南京农业大学学报》2018年第5期860-866,共7页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(41671294)

摘　　要：[目的]本文旨在研究一种纳米铁氧化物(水铁矿)对玉米相关生理生化指标和抗氧化系统的影响,为水铁矿作为新型铁肥促进玉米生长提供理论依据。[方法]以去离子水(CK1)为对照,用不同质量浓度(10、20、50、100、200和500 mg·L-1)水铁矿溶液进行浸种试验。以不含铁的1/2 Hoagland营养液(CK2)和含EDTA-Fe的1/2 Hoagland营养液(CK3)为2组对照,分别以不含铁的1/2 Hoagland营养液配制不同质量浓度(10、20、50、100、200和500 mg·L-1)的水铁矿溶液进行玉米幼苗培养试验,测定其相关生理生化指标并观察其细胞显微结构。[结果]玉米种子萌发试验结果表明:水铁矿质量浓度为20 mg·L-1时,玉米种子的萌发指数、活力指数和平均根长分别比CK1增加了38.33%、61.99%和53.91%,平均发芽时间缩短56.63%。玉米幼苗培养试验结果表明:水铁矿质量浓度为20 mg·L-1时地上部鲜质量、株高和叶绿素含量分别比CK2显著增加了15.38%、10.03%和24.85%,水铁矿质量浓度高于50 mg·L-1时不利于玉米生长。水铁矿溶液质量浓度低于20 mg·L-1时玉米叶片中过氧化物酶(POD)和过氧化氢酶(CAT)活性逐渐升高,高于此浓度时POD和CAT活性逐渐降低。地上部铁含量检测和透射电镜观察结果显示:水铁矿质量浓度为10 mg·L-1时,水铁矿颗粒能够进入到玉米根细胞内,且地上部铁含量比CK2显著提高了75%,但水铁矿质量浓度大于20 mg·L-1时会造成根细胞质壁分离。[结论]低质量浓度(10~20 mg·L-1)水铁矿可替代EDTA-Fe,有助于玉米种子萌发和植株生长,而高质量浓度(≥50 mg·L-1)水铁矿不利于玉米生长。[Objectives]The research was aimed to study the effect of a nano-iron oxide(ferrihydrite)on maize growth and antioxidant system and to provide theory basis for ferrihydrite used as a new iron fertilizer on maize growth.[Methods]Deionized water(CK1)was set up as control while a series of ferrihydrite,i.e.10,20,50,100,200 and 500 mg·L-1 were used as treatments during seed germination.Then seedlings were transferred to 1/2 Hoagland solution without iron(CK2)and with EDTA chelated iron(CK3)while a series of ferrihydrite,i.e.10,20,50,100,200 and 500 mg·L-1 with CK2 solution were used as treatments.The biological and physiological indicators as well as cell micro-morphological structures were determined during maize seed germination and seedling growth.[Results]The seed germination experiment results showed that the germination index,the vigor index and the average root length increased significantly by 38.33%,61.99%and 53.91%,respectively,and the average germination time reduced by 56.63%,when the concentration of ferrihydrite was 20 mg·L-1 as compared with CK1.The seedling growth experiment results showed that the fresh weight of shoot,root of length and chlorophyll content increased significantly by 15.38%,10.03%and 24.85%as compared with the CK2,when the concentration of ferrihydrite was 20 mg·L-1.Concentration higher than 50 mg·L-1 had damaged maize growth.Ferrihydrite concentrations of less than 20 mg·L-1 increased POD and CAT activities in maize leaves.However,the concentrations of more than 20 mg·L-1 led to the gradual reduction of POD and CAT activities.In addition,results of leaf Fe determination and transmission electron microscopy observation showed that ferrihydrite at the concentration of 10 mg·L-1 could enter the root cells of maize and significantly increased the content of iron in shoot by 75%as compared with CK2.However,higher ferrihydrite concentration of more than 20 mg·L-1 resulted in the plasmolysis of root cell.[Conclusions]The promoting effect of ferrihydrite on seed germination and gro

关 键 词：水铁矿 玉米 生理生化 抗氧化系统 质壁分离 

分 类 号：S145.5[农业科学—肥料学]