miR-107与非小细胞肺癌的临床病理特征及预后的关联  

Circulating miR-107 Expression Correlates with the Clinicopathological Features and Prognosis of Patients with Non-small Cell Lung Cancer

作　　者：赵轲 张永建 刘奇 徐迪 陈宝钧[1] ZHAO Ke;ZHANG Yongjian;LIU Qi;XU Di;CHEN Baojun(Department of Thoracic Surgery,The Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430014,China)

机构地区：[1]华中科技大学同济医学院附属武汉中心医院胸外科,武汉430014

出　　处：《中国医科大学学报》2018年第10期933-938,共6页Journal of China Medical University

摘　　要：目的评估血浆miR-107表达水平与非小细胞肺癌(NSCLC)患者的临床病理特征及预后的关联。方法纳入196例NSCLC患者,采集外周血和肿瘤组织样本。抽提总RNA,采用实时PCR法检测miR-107的表达水平。结果 NSCLC患者中miR-107在血浆和肿瘤组织中的表达水平正相关(r=0.456,P <0.001);血浆miR-107表达水平与淋巴结转移(r=0.168,P=0.018)正相关,肿瘤组织miR-107与病理分化(r=0.304,P <0.001)、肿瘤大小(r=0.230,P=0.001)、淋巴转移(r=0.446,P <0.001)以及TNM分期(r=0.294,P <0.001)正相关;NSCLC患者的5年无病生存期(DFS)和总生存期(OS)分别为13.6%和32.8%;血浆和肿瘤组织中miR-107高表达均与较短的DFS与OS相关;单元Cox风险回归模型显示,血浆和肿瘤组织中miR-107高表达可预测患者较短的DFS和OS,进一步采用多元Cox风险回归模型分析,结果表明,血浆miR-107高表达(P=0.005)仅可独立预测患者的OS,而肿瘤组织中miR-107高表达可独立预测患者的DFS及OS (均P <0.001)。结论循环miR-107可作为新颖可靠的生物标记物用于评估NSCLC患者的临床病理特征和预后情况。Objective To evaluate the association of plasma microRNA-107(miR-107)expression with the clinicopathological properties and prognosis in non-small cell lung cancer(NSCLC)patients.Methods Plasma and tumor tissue samples were collected from 196 NSCLC patients.Total RNA was extracted and miR-107 expression was evaluated by real-time polymerase chain reaction.Results Expression of miR-107 in plasma was positively associated with expression in tumor tissue(r=0.456,P<0.001)and with lymph node metastasis(LYM)(r=0.168,P=0.018),but was not correlated with other clinicopathological features.In the tumor tissue samples,miR-107 expression was positively correlated with pathological grade(r=0.304,P<0.001),tumor size(r=0.230,P=0.001),LYM(r=0.446,P<0.001),and TNM stage(r=0.294,P<0.001).The 5-year disease-free survival(DFS)and overall survival(OS)rates were 13.6%and 32.8%,respectively.Kaplan-Meier curves revealed the correlations of the high expression of plasma miR-107 with worse DFS(P=0.002)and OS(P<0.001)compared to that of low expression.High expression of miR-107 in tissue samples was associated with worse DFS(P<0.001)and OS(P<0.001).Univariate Cox proportional hazard regression analysis indicated correlations of high expression of miR-107 in plasma(P=0.003)and tissue(P<0.001)with worse DFS and OS.Multivariate Cox regression analysis revealed that high expression of miR-107 in plasma(P=0.005)might predict OS for NSCLC,while high expression of miR-107 in tumor tissue is an independent risk factor for shorter DFS and OS(both P<0.001).Conclusion Circulating miR-107 is a novel and potentially important prognostic biomarker in NSCLC patients.

关 键 词：血浆 miR-107 非小细胞肺癌 临床病理特征 预后 

分 类 号：R734.2[医药卫生—肿瘤]