五味子乙素对视网膜母细胞瘤移植癌裸鼠存活和VEGF表达的影响  

Effects of schisandrin B on survival and expression of VEGF in retinoblastoma implantation model in nude mice

作　　者：乔利峰[1] 董文韬[1] 李杰[1] 雷春涛[1] 曲超[1] 林伟[1] QIAO Li-Feng;DONG Wen-Tao;LI Jie;LEI Chun-Tao;QU Chao;LIN Wei(Department of Ophthalmology,Sichuan Academy of Medical Sciences&Sichuan Provincial People's Hospital,Chengdu 610072,China)

机构地区：[1]四川省医学科学院四川省人民医院眼科,成都610072

出　　处：《中国免疫学杂志》2018年第9期1326-1330,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金青年项目(No.81700841)

摘　　要：目的:探究五味子乙素对视网膜母细胞瘤移植癌裸鼠存活和血管内皮生长因子(VEGF)表达的影响。方法:裸鼠随机分为4组:移植瘤模型(Y79)组、五味子乙素低中高剂量(10、20、50 mg/kg BW)组。末端脱氧核苷酸转移酶介导的d UTP缺口末端标记法(TUNEL)染色,检测肿瘤组织细胞凋亡。免疫组化染色检测肿瘤组织中VEGF表达。蛋白印迹检测肿瘤组织中VEGFR2、P-P38、P38、P-Hsp27和Hsp27蛋白水平。结果:五味子乙素(10、20、50 mg/kg)组裸鼠肿瘤体积小于移植瘤模型组(P<0. 01)。同时,五味子乙素(10、20、50 mg/kg)组裸鼠存活率高于移植瘤模型组(P<0. 01)。与移植瘤模型组相比,五味子乙素(10、20、50 mg/kg)组肿瘤组织细胞凋亡升高(P<0. 01)。另外,五味子乙素(10、20、50 mg/kg)组肿瘤组织中VEGF表达低于移植瘤模型组(P<0. 01)。与移植瘤模型组相比,五味子乙素(10、20、50 mg/kg)组肿瘤组织VEGFR2蛋白水平及P-P38/P38和P-Hsp27/Hsp27比值下降(P<0. 01)。结论:五味子乙素可减弱视网膜母细胞瘤移植癌裸鼠肿瘤生长,提高存活率,降低VEGF表达,抑制VEGF信号通路活化。Objective:To explore the effects of schisandrin B on the survival and expression of vascular endothelial growth factor(VEGF)in retinoblastoma implantation model in nude mice.Methods:The nude mice were randomly divided into four groups,included implantation model(Y79)group,schisandrin B(10,20,50 mg/kg BW)groups.The apoptosis of tumor tissues was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick labeling(TUNEL)staining.The expression of VEGF of tumor tissues was measured by immunohistochemical staining.The protein levels of VEGFR2,P-P38,P38,P-Hsp27 and Hsp27 of tumor tissues were tested by Western blot.Results:The tumor volume in schisandrin B(10,20,50 mg/kg)groups was smaller than implantation model group(P<0.01).At the same time,the survival rate in schisandrin B(10,20,50 mg/kg)groups was higher than implantation model group(P<0.01).Compared with implantation model group,the apoptosis of tumor tissues in schisandrin B(10,20,50 mg/kg)groups was elevated(P<0.01).In addition,the expression of VEGF of tumor tissues in schisandrin B(10,20,50 mg/kg)groups was lower than implantation model group(P<0.01).Compared with implantation model group,the protein levels of VEGFR2 and the rate of P-P38/P38 and P-Hsp27/Hsp27 were reduced(P<0.01).Conclusion:Schisandrin B attenuated tumor growth,enhanced survival rate,reduced expression of VEGF and inhibited activation of VEGF pathway.

关 键 词：视网膜母细胞瘤 五味子乙素 存活 细胞凋亡 VEGF信号通路 

分 类 号：R739.72[医药卫生—肿瘤]