瑞舒伐他汀对心肌缺血再灌注损伤大鼠心肌凋亡及相关基因表达的影响  

Effect of Rosuvastatin on myocardial cell apoptosis in rat model of myocardial ischemia reperfusion injury

作　　者：谢亚芹[1] 李浩 赵娟[1] 孟凡星[1] 崔海鹏[1] 李瑞香[1] Ya-qin Xie;Hao Li;Juan Zhao;Fan-xing Meng;Hai-peng Cui;Rui-xiang Li(Department of Pathophysiology,Chengde Medical College,Chengde,Hebei 067000,China;Chengde Central Blood Stations,Chengde,Hebei 067000,China)

机构地区：[1]承德医学院病理生理学教研室,河北承德067000 [2]河北省承德市中心血站,河北承德067000

出　　处：《中国现代医学杂志》2018年第27期10-14,共5页China Journal of Modern Medicine

基　　金：河北省卫生计生委医学科学研究课题(No:20170231);河北省高校重点学科建设项目(No:冀教高[2013]4号)

摘　　要：目的探讨瑞舒伐他汀对心肌缺血再灌注损伤(MIRI)大鼠心肌细胞凋亡及相关基因表达的影响。方法雄性Wistar大鼠随机分为假手术组(Sham组)、心肌缺血再灌注组(MIRI组)、心肌缺血再灌注+瑞舒伐他汀组(MIRI+R组),每组10只。复制大鼠MIRI模型,观察各组大鼠左心室舒张末压(LVEDP),左心室内压最大上升和下降速率(±dp/dtmax);脱氧核糖核苷酸末端转移酶介导的缺口末端标记法检测大鼠左心室心肌细胞的凋亡指数;免疫组织化学染色检测左心室心肌组织凋亡相关基因(Bcl-2、Bax)蛋白表达,RT-PCR法检测Bcl-2、Bax mRNA表达。结果 MIRI组与Sham组比较,大鼠LVEDP、心肌细胞凋亡指数(AI)、Bax蛋白及mRNA的表达升高(P<0.05),±dp/dtmax、Bcl-2蛋白及mRNA的表达、Bcl-2/Bax比值下降(P <0.05);与MIRI组比较,MIRI+R组大鼠LVEDP、AI、Bax蛋白及mRNA的表达降低(P <0.05),±dp/dtmax、Bcl-2蛋白及mRNA的表达、Bcl-2/Bax比值升高(P <0.05)。结论瑞舒伐他汀可降低心肌缺血再灌注损伤引起的心肌细胞凋亡,具有良好的心肌保护作用。Objective To investigate the effect of Rosuvastation on myocardial cell apoptosis and apoptosisassociated genes expression in rat model of myocardial ischemia reperfusion injury(MIRI).Methods MIRI model was constructed based on standard procedure.Rat in Sham group received all procedures except for myocardial infarction and reperfusion.Rosuvastation was administrated in treatment group.Hemodynamic parameters including LVEDP and±dp/dtmax were recorded.Cellular apoptotic index(AI)of left ventricular was measured by TUNEL staining.Apoptotic related Bcl-2 and Bax were measured by Immunohistochemistry and RT-PCR.Results Rats in MIRI group experienced increased levels of LVEDP,AI and Bax and decreased levels of±dp/dtmax and Bcl-2 when compared with Sham group(P<0.05).All the mentioned alteration of apoptotic status were reversed by treatment of Rosuvastationin MIRI+R group compared with MIRI group(P<0.05).Conclusion Rosuvastatin exerts myocardial protection through downregulation of cell apoptosis in rat model of myocardial ischemia reperfusion injury.

关 键 词：瑞舒伐他汀 心肌缺血再灌注损伤 细胞凋亡 凋亡相关基因 

分 类 号：R541.4[医药卫生—心血管疾病]