MMP-9过表达对骨肉瘤143B细胞侵袭与迁移能力的影响  

Effect of MMP-9 over-expression on invasion and migration of osteosarcoma 143B cells

作　　者：齐巍[1] 李勇[2] 琚绍静 孙娜 张瑞杰[1] Wei Qi;Yong Li;Shao-jing Ju;Na Sun;Rui-jie Zhang(Department of Trauma,the Second Hospital of Tangshan,Tangshan,Hebei 063000,China;Department of Orthopedics,the Second Hospital of Tangshan,Tangshan,Hebei 063000,China;School of Basic Medical Sciences,North China University of Science and Technology,Tangshan,Hebei 063000,China)

机构地区：[1]河北省唐山市第二医院创伤一科,河北唐山063000 [2]河北省唐山市第二医院骨病科,河北唐山063000 [3]华北理工大学基础医学院,河北唐山063000

出　　处：《中国现代医学杂志》2018年第22期13-18,共6页China Journal of Modern Medicine

摘　　要：目的研究MMP-9过表达对成骨肉瘤143B细胞侵袭与迁移能力的影响。方法通过慢病毒介导MMP-9过表达载体转染骨肉瘤143B细胞,实验分为3组:实验组(转染p LV-Puro MMP-9过表达序列)、对照组(转染p LV-Puro MMP-9-NC序列)、空白组(PBS);实时荧光定量聚合酶链反应(q RT-PCR)检测MMP-9和VEGF m RNA的表达;Western blot法检测MMP-9和VEGF蛋白的表达;Transwell小室侵袭实验检测细胞侵袭能力;细胞划痕实验检测细胞迁移能力。结果实验组MMP-9和VEGF的m RNA和蛋白表达量较对照组和空白组上调(P<0.05);实验组穿过人工基底膜细胞数高于对照组和空白组(P<0.05);实验组划痕愈合率高于对照组和空白组(P<0.05)。结论慢病毒介导MMP-9过表达通过作用于VEGF靶基因,抑制成骨肉瘤143B细胞侵袭及迁移能力。Objective To study the effect of MMP-9 over-expression on invasion and migration of osteosarcoma 143B cells.Methods Lentivirus-mediated MMP-9 over-expression vector was transfected into osteosarcoma 143B cells.The experiment was divided into an experimental group(transfected with pLV-Puro MMP-9 over-expression sequence),a control group(transfected with pLV-Puro MMP-9-NC sequence)and a blank group(PBS).qRT-PCR was used to detect the expressions of MMP-9 and VEGF mRNAs.The expressions of MMP-9 and VEGF proteins were detected by Western blot.Transwell invasion assay was used to detect the invasion ability.Cell migration was measured by cell scratch test.Results The mRNA and protein expressions of MMP-9 and VEGF in the experimental group were significantly higher than those in the control and blank groups(P<0.05).The number of cells passing through the artificial basement membrane in the experimental group was significantly larger than that in the control group and the blank group(P<0.05).The healing rate of scratches in the experimental group was significantly higher than that in the control group and the blank group(P<0.05).Conclusions Lentivirus-mediated MMP-9 overexpression inhibits the invasion and migration of osteosarcoma 143B cells by acting on VEGF target gene.

关 键 词：基质金属蛋白酶9 血管内皮生长因子 骨肉瘤细胞 侵袭 迁移 

分 类 号：R738.1[医药卫生—肿瘤]