深低温冷冻保存血小板的最佳温度及时限  

Optimal temperature and storage time span for cryopreservation of platelets

作　　者：杨振宇[1] 李正发[2] 付凌梅 朱祥明[1] Yang Zhen-yu;Li Zheng-fa;Fu Ling-mei;Zhu Xiang-ming(Yunnan Kunming Blood Center,Kunming 650106, Yunnan Province,China;the First People’s Hospital of Yunnan Province,Kunming 650000,Yunnan Province,China)

机构地区：[1]云南昆明血液中心,云南省昆明市650106 [2]云南省第一人民医院,云南省昆明市650000

出　　处：《中国组织工程研究》2018年第33期5368-5372,共5页Chinese Journal of Tissue Engineering Research

基　　金：云南省第一人民医院奥新基金项目(2014DS006)~~

摘　　要：背景:临床对血小板的需求量急剧增加,新鲜液态血小板无法保障临床需求,特别是特殊血型、急诊患者和偏远地区的需求。目的:探讨用5%二甲基亚砜深低温冰冻保存血小板的最佳保存温度、时限及复融后的最佳使用时间。方法:选择16个机采血小板标本,对冰冻前及在-18℃、-40℃、-80℃冰冻保存1,3,6,12个月、冻融后即刻、冻融后1,2,4 h进行相关指标检测,包括血小板计数、平均血小板体积、血小板分布宽度、CD42b和CD62p表达。结果与结论:(1)-80℃冰冻保存6个月血小板的CD62p表达、冰冻保存12个月血小板的CD42b、CD62p表达分别与冰冻前比较差异有显著性意义(P <0.05);(2)-18℃、-40℃冰冻保存6个月血小板的CD42b、CD62p表达与-80℃相比差异均有显著性意义(P<0.05);(3)-80℃冰冻保存6个月复融后4hCD42b、CD62p表达与复融后即刻相比差异有显著性意义(P <0.05);(4)结果表明5%二甲基亚砜冰冻保存血小板在越低的温度下保存越好;推荐保存条件为-80℃、6个月内;解冻后的最佳使用时间为2 h内。BACKGROUND:Clinical demand of fresh liquid platelets cannot be guaranteed because of the rapid increase of the need of platelets,especially for the specific blood types,emergency patients and remote areas.OBJECTIVE:To explore the optimal temperature and storage time span for deep cryopreservation of platelets in 5%dimethyl sulfoxide as well as the optimal usage time after melting of the frozen platelets.METHODS:Sixteen platelet samples filtered by the machine were randomly selected.Indexes as blood platelet count,mean platelet volume,platelet distribution width,CD42b,CD62p were separately tested before and 1,3,6,12 months after cryopreservation at three different temperatures of-18,-40,and-80 oC as well as immediately,1,RESULTS AND CONCLUSION:Significant changes were found in the expression of CD62p in the platelets at 6 and 12 months after cryopreservation at-80 oC and the expression of CD42b in the platelets at 6 months after cryopreservation at-80 oC(P<0.05).The expression of CD42b and CD62p showed a significant difference in the platelets stored at-80 oC and at-18 and-40 oC for 6 months(P<0.05).For the platelets stored at-80 oC for 6 months,the expression of CD42b and CD62p in the platelets at 4 hours after melting was significantly different from that immediately after melting(P<0.05).These findings reveal that the lower temperature indicates the better cryopreservation of platelets using 5%dimethyl sulfoxide.The recommended storage conditions are-80 oC for 6 months;and the optimal usage time after melting is within 2 hours.

关 键 词：血小板 低温冰冻保存 保存温度 保存时限 二甲基亚砜 复融 CD42B CD62P 干细胞 血小板 低温保存 二甲亚砜 组织工程 

分 类 号：R394.2[医药卫生—医学遗传学]