丙泊酚对人胃癌裸鼠移植瘤生长的影响  

Effect of Propofol on growth of human gastric cancer xenografts in nude mice

作　　者：胡微澜[1] 韩威利 杜增利 Wei-lan Hu;Wei-li Han;Zeng-li Du(Xinxiang Central Hospital,Xinxiang,Henan 453000,China;Central Hospital of Jiaozuo Coal Industry Group,Jiaozuo,Henan 454150,China)

机构地区：[1]河南省新乡市中心医院,河南新乡453000 [2]河南省焦作煤业集团中央医院,河南焦作454150

出　　处：《中国现代医学杂志》2018年第29期19-24,共6页China Journal of Modern Medicine

摘　　要：目的探讨丙泊酚对人胃癌裸鼠移植瘤生长的影响,及其可能的作用机制。方法培养人胃癌细胞株SGC7901,复制裸鼠移植瘤模型30只,随机分为对照组、生理盐水组和丙泊酚组,每组10只。对照组未做任何处理;生理盐水组腹腔注射生理盐水1.5 ml/kg;丙泊酚组腹腔注射丙泊酚20 mg/kg,1次/d,连续2周。观察各组裸鼠给药前后一般情况的变化,给药后每3 d用游标卡尺测量移植瘤的长径和短径,计算移植瘤体积。第15天时将裸鼠脱臼处死,剥离瘤体。采用实时荧光定量聚合酶链反应和Western blot检测各组裸鼠移植瘤组织中核因子E2相关因子2(Nrf2)、NAD(P)H醌氧化还原酶1(NQO1)、血红素加氧酶-1(HO-1)、B淋巴细胞瘤(Bcl-2)、Bcl-2相关X蛋白(Bax)基因和蛋白的表达。结果丙泊酚组裸鼠给药后3、6、9、12和15 d的移植瘤体积小于对照组和生理盐水组(P<0.05)。与对照组和生理盐水组相比,丙泊酚组裸鼠移植瘤体质量降低,而抑瘤率升高(P <0.05)。与对照组和生理盐水组相比,丙泊酚组裸鼠移植瘤组织中Nrf2、NQO1、HO-1、Bcl-2 mRNA和蛋白相对表达量降低,而Bax mRNA和蛋白相对表达量升高(P<0.05)。结论丙泊酚可抑制人胃癌裸鼠移植瘤的生长,其机制可能与抑制Nrf2/ARE信号通路,从而促进细胞凋亡有关。Objective To investigate the effect of Propofol on growth of human gastric cancer xenografts in nude mice and the possible mechanisms.Methods Human gastric cancer cell line SGC7901 was cultured.The models of subcutaneous tumor were established in thirty BALB/c nude mice,and randomly divided into a control group,a saline group and a Propofol group.The mice in the control group did not receive any treatment,those in the saline group were given intraperitoneal injection of saline 1.5 ml/kg once a day for 2 consecutive weeks,while the mice in the Propofol group received Propofol 20 mg/kg once a day for 2 consecutive weeks.The changes of the general condition of the nude mice before and after administration were observed.The caliper was used to measure the tumor size every 3 days after administration,and the tumor volume was calculated.The nude mice were sacrificed on the 15th d,and the tumors were isolated.The expressions of nuclear factor E2-related factor 2(Nrf2),NAD(P)Hquinine oxidoreductase 1(NQO1),heme oxygenase-1(HO-1),B-cell lymphoma-2(Bcl-2),Bcl-2 associated X protein(Bax)genes and proteins in the tumor tissues of different groups were detected using qRT-PCR and Western blot,respectively.Results The tumor volume in the Propofol group was smaller than that in the control group and the saline group 3,6,9,12 and 15 d after administration,the differences were statistically significant(P<0.05).Compared with the control group and the saline group,the tumor weight was decreased,while the tumor inhibition rate was increased in the Propofol group,the differences were statistically significant(P<0.05).Compared with the control group and the saline group,the relative expression levels of Nrf2,NQO1,HO-1 and Bcl-2 mRNAs and proteins in the tumor tissues were decreased,while the relative expression levels of Bax mRNA and protein were increased in the Propofol group,the differences were statistically significant(P<0.05).Conclusions Propofol could inhibit the growth of human gastric cancer xenografts in nude mice.The mechani

关 键 词：胃癌 丙泊酚 移植瘤 Nrf2/ARE信号通路 

分 类 号：R735.2[医药卫生—肿瘤]