克里布所类芽孢杆菌6hRe76产木聚糖酶条件的优化  

Optimization of xylanase production conditions by Paenibacillus kribbensis 6hRe76

作　　者：马春玲[1] 李慧 李广硕 王佳硕 武永秀[1] 孙磊[1] MA Chun-ling;LI Hui;LI Guang-shuo;WANG Jia-shuo;WU Yong-xiu;SUN Lei(College of Life Sciences,Hebei University/Key Laboratory of Microbial Diversity Research and Application of Hebei,Baoding 071002,China)

机构地区：[1]河北大学生命科学学院/河北省微生物多样性研究与应用重点实验室,河北保定071002

出　　处：《河北农业大学学报》2018年第5期19-24,共6页Journal of Hebei Agricultural University

基　　金：国家自然科学基金(31100002);河北省生物工程重点学科经费

摘　　要：为了提高木聚糖酶产量,采用单因素试验和响应面分析法,对木聚糖酶产生菌克里布所类芽孢杆菌6hRe76产木聚糖酶的发酵培养基和发酵条件进行优化。利用单因素试验确定最佳碳源和最佳氮源分别为麸皮、酵母膏。然后利用PB试验筛选出影响木聚糖酶产量的主要因素为装液量、麸皮、K_2HPO_4,进一步利用Box-Behnken软件进行试验设计,并对试验数据处理得到响应模型。预测得到菌株6hRe76的最佳培养条件为:麸皮5.92%,K_2HPO_40.71%,酵母膏0.75%,MgSO_40.05%,CaCl_20.05%,吐温80 0.15%,装液量11mL/50mL三角瓶,接种量10%,种龄24h,34℃,初始pH9.0,培养时间36h。在最佳培养条件下,菌株6hRe76产酶量达92.11U/mL,比原始水平57.15U/mL提高了61.17%。In order to obtain high yield of xylanase,the fermentation medium and fermentation conditions on xylanase production by Paenibacillus kribbensis 6hRe76 were optimized by single-factor experiments and response surface methodology.The single-factor experiment was used to screen the best carbon source and nitrogen source.The results showed that wheat bran and yeast extract was as the optimum carbon source and nitrogen source,respectively.Wheat bran,K 2HPO 4 and liquid volume were certified as the major factors influencing xylanase production by Plackett-Burman experiment.Then,Box-Behnken was used in experimental design,experimental data were analyzed and the response surface mode constructed indicated that the optimal conditions for xylanase production by strain 6hRe76 were as following:wheat bran 5.92%,K 2HPO 4 0.71%,yeast extract 0.75%,MgSO 4 0.05%,CaCl 2 0.04%,Tween80 0.15%,liquid volume 11/50 mL,inoculum 10%,seed age 24h,34℃,initial pH 9.0,cultured for 36 h.Under the optimal conditions,the maximum activity of xylanase was 92.11U/mL.The xylanase production of 6hRe76 increased 61.17%than the original level of 57.15 U/mL.

关 键 词：木聚糖酶 响应面优化 克里布所类芽孢杆菌 

分 类 号：Q939.13[生物学—微生物学]