c-Myc调控的长链非编码RNA lncRNA55对舌鳞癌细胞生长的影响  

Effect of long non-coding RNA lncRNA55 regulated by c-Myc on the growth of tongue squamous cell carcinoma cells

作　　者：韩曈曈 徐艳雪 朱友明[1,2] 陈乔尔[1,2] Han Tongtong;Xu Yanxue;Zhu Youming(Stomatologic College of Anhui Medical University,Hefei 230032;The Affiliated Stomatological Hospital of Anhui Medical University,Key Laboratory of Oral Diseases Research of Anhui Province,Hefei 230032)

机构地区：[1]安徽医科大学口腔医学院,合肥230032 [2]安徽医科大学附属口腔医院安徽省口腔疾病研究中心实验室,合肥230032

出　　处：《安徽医科大学学报》2018年第11期1700-1705,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1508085MH155)

摘　　要：目的阐明长链非编码RNA(lncRNA) lncRNA55与癌基因c-Myc表达之间的相关性,并探讨敲低lncRNA55对舌鳞癌细胞SCC3生长的影响。方法将携带flag-c-Myc和plko. 1-shc-Myc的慢病毒载体分别转染SCC3细胞,Western blot和实时定量PCR (qRT-PCR)检测c-Myc蛋白与lncRNA55的表达。在此基础上构建shlncRNA55的表达载体并转染SCC3,qRT-PCR检测转染效率,MTT法和集落形成实验检测SCC3细胞生长情况。此外,原位杂交免疫荧光实验检测了lncRNA55在SCC3细胞内的分布。结果 c-Myc敲低可以使lncRNA55表达明显上调而c-Myc过表达可以使lncRNA55下调; shlncRNA55可有效降低lncRNA55的表达;降低lncRNA55表达能显著促进SCC3细胞增殖和集落形成;并发现lncRNA55主要存在细胞质中。结论 LncRNA55受c-Myc负调控,敲低SCC3中lncRNA55表达能促进细胞增殖。Objective To illuminate the correlation between the expression of long non-coding RNA(lncRNA)lncRNA55 and oncogene c-Myc,and investigate the effect of lncRNA55 knockdown on the growth of SCC3 tongue squamous cell carcinoma cells.Methods Infecting SCC3 cells with lentiviral vector carrying flag-c-Myc or plko.1-shc-Myc,then detect the expression of c-Myc protein and lncRNA55 by Western blot analysis and real-time quantitative PCR(qRT-PCR).Based on this,vector carrying shlncRNA55 was constructed and transfected into SCC3 cells,the transfection efficiency was evaluated by using qRT-PCR.Then the cell proliferation of SCC3 was detected by MTT and colony formation assays.In addition,we explored the subcellular distribution of lncRNA55 by fluorescence in situ hybridization.Results The knockdown of c-Myc could significantly increase the expression of lncRNA55,while the overexpression of c-Myc can downregulate lncRNA55.shlncRNA55 could effectively reduce the expression of lncRNA55;decreasing lncRNA55 expression could significantly promote the proliferation and colony formation of SCC3 cells;and lncRNA55 was mainly found in the cytoplasm.Conclusion LncRNA55 may be negatively regulated by c-Myc.LncRNA55 knockdown can promote SCC3 cell proliferation.

关 键 词：舌鳞癌 长链非编码RNA C-MYC LncRNA55 细胞增殖 

分 类 号：R739.8[医药卫生—肿瘤]