PBX3基因通过p38信号通路对子宫内膜癌细胞生物学特性的影响研究  

Effects of PBX3 gene on biological characteristics of endometrial cancer cells through p38 signaling pathway

作　　者：丁文清[1] 袁迎九[1] 后建丽[2] 梁政巧 Ding Wenqing;Yuan Yingjiu;Hou Jianli(Department of Gynecology,Shenzhen Longgang District Maternal and Child Health Hospital,Shenzhen 518172;Department of Obstetrics,Shenzhen Longgang District Maternal and Child Health Hospital,Shenzhen 518172)

机构地区：[1]深圳市龙岗区妇幼保健院妇科,深圳518172 [2]深圳市龙岗区妇幼保健院产科,深圳518172 [3]深圳市龙岗区妇幼保健院超声科,深圳518172

出　　处：《现代妇产科进展》2018年第10期741-744,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨前B细胞白血病同源盒基因3(PBX3)对子宫内膜癌细胞活力和凋亡率的影响及p38信号通路调控作用。方法:将靶向抑制PBX3的小干扰RNAs(siRNA-PBX3)及阴性对照siRNA(siRNA-Ctrl)转染人子宫内膜癌Ishikawa细胞,SB203580作为p38信号通路抑制剂,未转染细胞为空白对照组,转染48h。Western blot法检测PBX3、PCNA、Bax、p38和p-p38蛋白表达; MTT及流式细胞术检测细胞活力及凋亡率。结果:siRNA-PBX3转染可明显降低PBX3表达,与空白对照组比较,差异有统计学意义(P <0.05)。siRNA-PBX3组转染24h、48h和72h的细胞活力均降低,与siRNA-Ctrl组比较,差异均有统计学意义(P<0.05)。siRNA-PBX3转染细胞48h的细胞凋亡率升高,PCNA和p-p38蛋白表达降低,Bax蛋白表达升高,与siRNA-Ctrl组比较,差异均有统计学意义(P<0.05)。与siRNA-PBX3组和SB203580组比较,siRNA-PBX3+SB203580组的细胞活力均明显降低,凋亡率升高,差异均有统计学意义(P<0.05)。结论:PBX3基因表达抑制可通过下调p38信号通路降低子宫内膜癌细胞活力和诱导凋亡。Objective:To investigate the effect of PBX3 gene on cell viability and apoptosis rate of endometrial carcinoma and the regulation role of p38 signaling pathway.Methods:The small interference RNAs for targeted inhibition of PBX3(siRNA-PBX3)and negative control group siRNA(siRNA-Ctrl)were transfected into human endometrial carcinoma Ishikawa cells,SB203580 was used as a signal pathway inhibitor of p38 signaling pathway,the untransfected cells were the blank control group.Cells were transfected for 48h,Western blot was used to detect the proteins expressions of PBX3,PCNA,Bax,p38 and p-p38.MTT and flow cytometry were used to detect cell viability and apoptosis rate.Results:siRNA-PBX3 transfection significantly decreased the expression of PBX3,and the difference was statistically significant compared with the blank control group(P<0.05).Cell viability decreased in siRNA-PBX3 group transfected with 24h,48h and 72h,and the difference was statistically significant compared with siRNA-Ctrl group(P<0.05).The apoptosis rate increased cells were transfected for 48h in siRNA-PBX3 group,the expressions of PCNA and p-p38 protein decreased,and the expression of Bax protein increased,the difference was statistically significant compared with siRNA-Ctrl group(P<0.05).Compared with siRNA-PBX3 group or SB203580 group,the cell viability was significantly decreased and the rate of apoptosis increased in siRNA-PBX3+SB203580 group,and the difference was statistically significant(P<0.05).Conclusion:Inhibition of PBX3 gene expression can reduce cell viability and induce apoptosis in endometrial cancer cells by downregulating of the p38 signaling pathway.

关 键 词：子宫内膜癌 PBX3基因 P38信号通路 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]