p53通过调控长链非编码RNA lncRNAp53urlnc对舌鳞癌细胞的影响  

Effect of p53 on tongue squamous cell carcinoma cells through regulation of long chain noncoding RNA lncRNAp53urlnc

作　　者：周洁 朱友明[1,2] 邹多宏[1,2] Zhou Jie;Zhu Youming;Zou Duohong(Stomatologic College of Anhui Medical University,Hefei 230032;The Affiliated Stomatological Hospital of Anhui Medical University,Key Laboratory of Oral Diseases Research of Anhui Province,Hefei 230032)

机构地区：[1]安徽医科大学口腔医学院,合肥230032 [2]安徽医科大学附属口腔医院,安徽省口腔疾病研究中心实验室,合肥230032

出　　处：《安徽医科大学学报》2018年第10期1489-1493,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金青年基金(编号：31501103)

摘　　要：目的研究长链非编码RNA(lncRNA)与p53的表达之间的关系以及敲低lncRNAp53urlnc对舌鳞癌细胞(SCC3)的影响。方法在SCC3细胞中分别敲低和过表达p53后,分别提取各组RNA和蛋白,Western blot法检测p53蛋白的表达,实时定量PCR(qRT-PCR)技术检测lncRNAp53urlnc的表达。构建shlncRNAp53urlnc载体,包装病毒,并感染SCC3细胞,qRT-PCR检测转染效率,采用MTT法、集落形成试验检测shlncRNAp53urlnc对SCC3细胞生长影响。结果 p53敲低使lncRNAp53urlnc表达明显下调,p53过表达使lncRNAp53urlnc上调;敲低lncRNAp53urlnc表达促进SCC3细胞增殖。结论 p53通过正调控lncRNAp53urlnc抑制SCC3细胞增殖。Objective To investigate the relationship between the long non-coding RNA(lnc RNA)and the expression of p53,and the effect of knocking down lncRNAp53urlnc in tongue squamous cell carcinoma(SCC3).Methods The expression of p53 in SCC3 cells was knocked down and overexpressed,and then the RNA and protein of each group was collected.The expression of p53 protein was detected by Western blot and the expression of lncRNAp53urlnc was detected by real-time quantitative PCR(qRT-PCR).shlncRNAp53urlnc lentiviral vector was constructed,and then shlncRNAp53urlnc lentiviral vector was transfected into SCC3 cells,the efficiency of the vector was detected by qRT-PCR.The proliferation of SCC3 cells was dectected by MTT and colony forming assay.Results p53 knockdown reduced the expression of lncRNAp53urlnc significantly and p53 overexpression significantly increased lncRNAp53urlnc.Knockdown the expression of lncRNAp53urlnc can promote the proliferation of SCC3 cell.Conclusion lncRNAp53urlnc inhibits the proliferation of SCC3 cell,which is positive regulated by p53.

关 键 词：长链非编码RNA lncRNAp53urlnc P53 

分 类 号：R739[医药卫生—肿瘤] R730[医药卫生—临床医学]