去甲肾上腺素转运蛋白调控人结肠癌细胞迁移能力的研究  

Regulation of cell migration by norepinephrine transporter in human colon cancer cells

作　　者：张华华 赵小鸽 缪吉玉 侯妮[2] 刘利英[2] 张静[1] 韩继明[1] ZHANG Huahua;ZHAO Xiaoge;MIAO Jiyu;HOU Ni;LIU Liying;ZHANG Jing;HAN Jiming(Medical College,Yan′an University,Yan′an 716000;School of Basic Medical Science,Xi′an Jiaotong University, Xi′an 710061,China;Biomedical Research Experiment Center,Xi′an Jiaotong University,Xi′an 710061,China)

机构地区：[1]延安大学医学院,陕西延安716000 [2]西安交通大学基础医学院,陕西西安710061 [3]西安交通大学生物医学研究实验中心,陕西西安710061

出　　处：《西北大学学报（自然科学版）》2018年第5期693-698,共6页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(81760510);陕西省自然科学基金资助项目(2016JM3027;2014JM2-3032);延安大学研究生教育创新基金资助项目(YCX201710)

摘　　要：研究去甲肾上腺素转运蛋白(Norepinephrine Transporter,NET)在结肠癌中的作用。利用TCGA数据库分析NET与临床结肠癌患者的TNM及stage分期的关系;收集临床标本行NET免疫组化实验;在结肠癌细胞中下调NET的表达行Transwell实验和Western Blot观察其变化。临床生物大数据及临床标本免疫组化结果显示:NET与结肠癌患者的转移及分期呈正相关,下调NET的表达显著抑制人结肠癌细胞的迁移能力以及迁移相关分子MMP9的表达,去甲肾上腺素转运蛋白参与对人结肠癌细胞迁移能力的调控。To explore the role of norepinephrine transporter(NET)in colon cancer.The relationship between NET and TNM and stage of patients with colon cancer was analyzed using TCGA database.The clinical samples of colon cancer were collected and prepared for immunohistochemistry assay of NET.Changes of migration and proteins were detected by Transwell assay and Western Blot in NET-downregulated human colon cancer cells.Clinical biologic data and immunohistochemical results of clinical specimens showed a positive correlation between NET and metastasis and staging of colon cancer patients.Down-regulation of NET expression significantly inhibited the migration of human colon cancer cells and the expression of the migration-associated molecule MMP9.NET might play a critical role in the regulation of migration ability of human colon cancer cells.

关 键 词：去甲肾上腺素转运蛋白 人结肠癌细胞 迁移 

分 类 号：R735.3[医药卫生—肿瘤]