基于转录组学筛选与哺乳动物睾丸发育相关基因及调控网络的研究进展  

Progress of Screening Genes and Regulating Network Related to Testis Development in Mammals Based on Transcriptomics

作　　者：孙武[1] 罗静 李发弟[1,2] 李万宏 乐祥鹏[1] SUN Wu;LUO Jing;LI Fa-di;LI Wan-hong;YUE Xiang-peng(State Key Laboratory of Grassland Agro-ecosystems,Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China;Sheep Breeding Biotechnology Engineering Laboratory of Gansu Province,Minqin 733300,China)

机构地区：[1]兰州大学草地农业生态系统国家重点实验室,兰州大学农业农村部草牧业创新重点实验室,兰州大学草地农业科技学院,兰州730020 [2]甘肃省肉羊繁育生物技术工程实验室,民勤733300

出　　处：《畜牧兽医学报》2018年第9期1810-1817,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑计划(2015BAD03B05)；甘肃省科技重大专项计划(1602NKDH020)；甘肃省农业生物技术专项(GNSW-2015-24；GNSW-2016-22)；甘肃省农业科技创新项目(GNCX-2014-41)；长江学者和创新团队发展计划资助(IRT13019)

摘　　要：睾丸正常发育是雄性哺乳动物精子发生及有效繁殖力的先决条件,其发育过程极其精细和复杂,受到许多因素的影响及调控,但遗传调控占主导地位。哺乳动物睾丸的发育首先会受到大量蛋白编码基因的严格调控,非编码RNA(miRNA、lncRNA和piRNA)也在睾丸发育及精子发生过程扮演重要角色。近年来,以mRNA、miRNA、lncRNA、piRNA为主的转录组学在解析哺乳动物睾丸发育以及精子发生分子机制得到充分应用,并且取得了很大进展。本文从睾丸发育相关mRNA、miRNA、lncRNA、piRNA等方面对哺乳动物睾丸发育研究现状进行介绍与阐述,旨在为深入挖掘睾丸发育基因组变化以及畜牧业中动物育种提供重要理论依据。Testis maturation and development are a prerequisite for spermatogenesis and effective reproduction in mammals,the process of maturation is extremely complex,which was affected by multiple factors,especially genetic factors.Testis development process was strictly regulated by lots of protein-coding genes,while growing evidences showed that non-coding RNAs(miRNAs,lncRNAs and piRNAs)also played an important roles in testicular development and spermatogenesis.In recent years,the study of transcriptome based on mRNA,miRNA,lncRNA and piRNA have been successfully applied in exploring molecular mechanism of mammalian testicular development and spermatogenesis,and have made great progress.In this review,we summarized the current status of testis development research in mammals from the aspect of testicular developmental-related genes,miRNAs,lncRNAs and piRNA in order to provide reference and theoretical basis for understanding global genome expression change in testicular development and better guide in animal breeding in the livestock industry.

关 键 词：mRNA MIRNA lncRNA PIRNA 睾丸发育 精子生成 

分 类 号：S814[农业科学—畜牧学]