毛序棘豆内生真菌分离鉴定及系统进化分析  

Isolation,Identification and Phylogenetic Analysis of Fungal Endophyte in Oxytropis trichophora

作　　者：孙璐 任祯慧 宋润杰[1] 权海云 焦韵洁 黄竹涛 王敬龙[2] 赵宝玉[1] 路浩[1] SUN Lu;REN Zhen-hui;SONG Run-jie;QUAN Hai-yun;JIAO Yun-jie;HUANG Zhu-tao;WANG Jing-long;ZHAO Bao-yu;LU Hao(College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China;Institute of Pratacultural Science,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850000,China)

机构地区：[1]西北农林科技大学动物医学院,杨凌712100 [2]西藏农牧科学院草业科学研究所,拉萨850000

出　　处：《畜牧兽医学报》2018年第9期2025-2035,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31201958);陕西省自然科学基金重点项目(2017JZ004)

摘　　要：为探明毛序棘豆中内生真菌的种属及种群分布情况,对采集于甘肃省通渭县毛序棘豆样品,运用表面消毒法消毒样品后进行内生真菌分离,采用形态学和ITS序列分析技术对所得菌株进行种属鉴定,并通过MEGA5.05软件进行系统进化分析。结果显示,从毛序棘豆(根、茎、叶)中共分离得到内生真菌29株,经鉴定,分属于4纲、5目、6科、10属;其中茎的分离率(39.13%)最高,根(23.91%)次之,叶(11.11%)最少;而链格孢属(Alternaria sp.)和镰刀菌属(Fusariumsp.)是毛序棘豆内生真菌的优势菌属,其相对分离频率分别为27.59%和17.24%;木霉菌属(Trichodermasp.)为毛序棘豆内分布最广泛的菌属,在根、茎、叶中均有分布;将所得菌株作系统进化分析,并根据其亲缘关系远近分为Ⅰ和Ⅱ两大种群。本试验结果表明,毛序棘豆内生真菌数量、种类与种属分布存在明显组织差异,茎是内生真菌侵染和定植的主要部位且真菌多样性最高。To explore the species and population distribution of endophytic fungi from Oxytropis trichophora,Oxytropis trichophora samples in Tongwei county of Gansu province were collected,then were sterilized by using the method of surface disinfection.After that,endophytic fungi were insolated,and then the morphology techniques and ITS sequence analysis were used to identify their species,The phylogenetic relationship of endophytic fungi was analyzed by MEGA 5.05 software.The results showed that 29 species of endophytic fungi were isolated from Oxytropis trichophora belonging to 4 classes,5 orders,6 families and 10 genera,the isolation rate of stem was the highest(39.13%),followed with root(23.91%)and leaf was the least(11.11%).Alternaria sp.and Fusarium sp.were the dominant species of endophytic fungi,relative frequency(RF)were 27.59%and 17.24%,respectively.Trichoderma sp.was the most widely distributed in roots,stems and leaves of Oxytropis trichophora.The phylogenetic analysis of endophytic fungi isolated from Oxytropis trichophora showed that they were divided into two groups according to their genetic relationship.The result indicated there was an obviously difference in quantity,species and population distribution of endophytic fungi between different parts of Oxytropis trichophora.Stem was the most vulnerable to infection and colonization by endophytic fungi,and the diversity was the maximum in stem of Oxytropis trichophora.

关 键 词：毛序棘豆 内生真菌 分离鉴定 种群分布 系统进化 

分 类 号：S856.9[农业科学—临床兽医学]