热碱法提取异养小球藻蛋白质工艺优化  

Optimization of protein extraction from Heterotrophic chlorella using thermo-alkaline

作　　者：胡守珍 毕生雷[1,2] 黄丽丽 邹婷婷 郭乐乐[2] 杨迪 张乃群[1] HU Shou-zhen;BI Sheng-lei;HUANG Li-li;ZOU Ting-ting;GUO Le-le;YANG Di;ZHANG Nai-qun(College of Life Science and Technology,Nanyang Normal University,Nanyang 473061,China;State Key Laboratory of Motor Vehicle Biofuel Technology,Henan Tianguan Group Co.,Ltd.,Nanyang 473000,China)

机构地区：[1]南阳师范学院生命科学与技术学院,河南南阳473061 [2]河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,河南南阳473000

出　　处：《食品与发酵工业》2018年第9期212-217,共6页Food and Fermentation Industries

基　　金：河南省科技攻关计划(162102310259);"十二五"农村领域国家科技支撑计划(2011BAD14B05)

摘　　要：在异养小球藻提油之前提取蛋白质,为降低产油成本奠定基础。以蛋白质提取率为指标,使用热碱法提取小球藻中的蛋白质,首先采用单因素试验确定提取过程中主要影响因素的最佳水平,然后利用正交试验对主要因素的相互作用进行研究。结果表明,小球藻在Na OH添加量为20%(质量分数),液料比为50∶1,提取时间为60 min,提取温度为55℃的条件下蛋白质的提取率最高,为55. 24%。热碱法提取异养小球藻蛋白,提取率高、操作简单、成本低,具有较好的工业化应用前景,为异养小球藻产油技术的经济最大化提供了参考。Protein extraction from Heterotrophic chlorella before extracting oil can reduce its oil extraction cost.In this study,the protein extraction rate was used as an index,the hot alkali method was used to extract protein from Heterotrophic chlorella.Single factor test was used to determine the best level of main factors in the extraction process.Then the interaction of main factors was studied by orthogonal test.The results showed that the best extracting conditions were:NaOH 20%,solid-liquid ratio 50∶1,extraction time 60 min and extraction temperature 55℃.Under these conditions,the protein extraction rate of Heterotrophic chlorella was the highest of 55.24%.By hot alkali method,the extraction rate was high,the operation was simple and the cost was low.It has a good potential in industrial application.This study provides a reference for an economic maximization of Heterotrophic chlorella oil production.

关 键 词：异养小球藻 蛋白质 提取率 工艺优化 

分 类 号：TS201.21[轻工技术与工程—食品科学]