黏膜相关淋巴组织淋巴瘤中Ig蛋白重链表达与IgH基因重排的研究  

Heavy chain expression of Ig protein and rearrangement of IgH gene in mucosa-associated lymphoid tissue lymphoma

作　　者：林海月 王红霞 胡东东 陈昊 LIN Haiyue;WANG Hongxia;HU Dongdong;CHEN Hao(Department of Pathology,First People′s Hospital of Lianyungang Lianyungang Hospital Affiliated to Xuzhou Medical University,Lianyungang,Jiangsu 222002,China;Obstetrics and Gynecology,First People′s Hospital of Lianyungang Lianyungang Hospital Affiliated to Xuzhou Medical University,Lianyungang,Jiangsu 222002,China)

机构地区：[1]江苏省连云港市第一人民医院/徐州医科大学附属连云港医院病理科,江苏连云港222002 [2]江苏省连云港市第一人民医院/徐州医科大学附属连云港医院妇产科,江苏连云港222002

出　　处：《重庆医学》2018年第28期3663-3667,共5页Chongqing medicine

摘　　要：目的探索黏膜相关淋巴组织淋巴瘤(MALToma)中Ig蛋白重链表达及IgH基因重排对其与反应性淋巴组织增生(RLH)鉴别诊断的价值,以期找到有助于两者鉴别诊断的新方法。方法收集30例MALToma、10例滤泡性淋巴瘤(FL)、10例浆细胞瘤(PC)作为试验组,并收集10例RLH作为对照组。应用免疫组织化学和Biomed-2引物系统分别检测两组Ig蛋白重链表达及IgH基因重排情况。结果 Ig蛋白重链在两组表达模式有3种:单种Ig重链表达(模式1)、全阴性Ig重链缺失(模式2)及多种Ig重链表达(模式3)。3种肿瘤及试验组中模式1/2及IgH基因单克隆重排检出率均高于对照组(P<0.05),且两者联合检测的阳性率显著升高。结论单种Ig重链/全阴性Ig重链缺失表达的检出可以作为B细胞恶性淋巴瘤尤其是MALToma的诊断线索;联合Ig重链及IgH基因重排检测有助于MALToma的鉴别诊断。Objective To explore the value of the heavy chain of Ig protein and IgH rearrangement in the differential diagnosis of mucosa-associated lymphoid tissue lymphoma(MALToma)and reactive lymphoid tissue hyperplasia(RLH),in order to find a new method to distinguish them.Methods Thirty cases of MALToma,10 cases of follicular lymphoma(FL)and 10 cases of plasmacytoma(PC)were collected as experimental group and 10 cases of RLH were collected as control group.Immunohistochemistry and BIOMED-2 primer system were used to detect the expression of the heavy chain of Ig protein and the rearrangement of IgH gene,respectively.Results There were three patterns of the heavy chain of Ig protein expression in the two groups:a single type expression,all negative expression,and a various type expression.The positive rate of a single type/all negative expression of heavy chain and monoclonal gene rearrangement of IgH were higher in the three tumors and the experimental group than in the control group(P<0.05),and the joint detection of them significantly improved the positive rate.Conclusion The detection of a single type/all negative of heavy chain expression can be used as a diagnostic clue of B cell malignant lymphoma,especially MALToma.Combined with the detection of the heavy chain of Ig protein and IgH gene rearrangement can help the differential diagnosis of MALToma.

关 键 词：淋巴瘤 B细胞 边缘区 免疫球蛋白类 基因重排 

分 类 号：R733[医药卫生—肿瘤]