基于蓝光光盘技术的黄曲霉毒素B1快速定量检测  

Quantitative Detection of Aflatoxin B1 Based on Blu-Ray Disc Technology

作　　者：王海荣 张校亮 谭慷 李晓春 WANG Hairong;ZHANG Xiaoliang;TAN Kang;LI Xiaochun(Key Laboratory of Advanced Transducers and Intelligent Control System Ministry of Education and Shanxi Province, College of Physics and Optoelectronics,Taiyuan University of Technology,Taiyuan 030024,China)

机构地区：[1]新型传感器与智能控制教育部与山西省重点实验室,太原理工大学物理与光电工程学院,太原030024

出　　处：《传感技术学报》2018年第9期1307-1310,1315,共5页Chinese Journal of Sensors and Actuators

基　　金：国家自然科学基金项目(21505098,81741095,21575098,61775157);山西省平台基地和人才专项-优秀人才科技创新项目(201605D211033)。

摘　　要：提出了基于蓝光光盘BD(Blu-ray Disc)技术和间接竞争免疫反应原理的黄曲霉毒素B1(Aflatoxin B1,AFB1)的定量检测方法。文中采用Na OH溶液活化水解BD表面使生物分子固定在其表面,并结合二甲基硅氧烷(PDMS)微流控通道制备间接竞争免疫检测结构,通过纳米金标记/银染技术增强信号,利用标准光驱结合光盘数据质量诊断软件(PlexUtilities)分析光盘上反应条带的错误数与AFB1浓度之间的关系,实现了对AFB1的定量检测。同ELISA的检测结果进行对比,二者结果基本一致。该方法检测限达到0.5 ng/mL,检测范围为0.5 ng/mL^200 ng/mL,满足我国国标对食品中AFB1的限定标准。A method of detecting Aflatoxin B1(AFB1)based on Blu-ray technology and indirect competitive immunoassay was developed.The surface of off-the-shelf Blu-ray discs(BDs)was activated by NaOH and adapted as substrates for preparation of immunoassays.The assays were quantified by using unmodified Blu-Ray drive combined with a free disc-quality check program.The performance of the BDs based detection system was tested for the quantitative detection of AFB1,the result was consistent with that of the commercial ELISA.The limit of detection achieved was as low as 0.5 ng/mL and the dynamic response range is 0.5 ng/mL^200 ng/mL,which meets the standards set by authorities in China.

关 键 词：生化传感器 黄曲霉毒素B1 蓝光技术 光电检测 间接竞争免疫检测 现场快速检测 

分 类 号：TP393[自动化与计算机技术—计算机应用技术]