一株产天然蓝色素细菌的分离鉴定  

Isolation and Identification of a Bacterium Producing Natural Blue Pigment

作　　者：肖咪云 阮楚晋 陈寿昆 刘裕华 陆祖军[1,2] XIAO Miyun;RUAN Chujin;CHEN Shoukun;LIU Yuhua;LU Zujun(College of Life Science,Guangxi Normal University,Guilin Guangxi,541006,China;Key Laboratory of Ecological and Environmental Protection for Rare and Endangered Animals and Plants(Guangxi Normal University),Guilin Guangxi 541006,China;Institute of Microbiology, Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]广西师范大学生命科学学院,广西桂林541006 [2]珍稀濒危动植物生态与环境保护重点实验室(广西师范大学),广西桂林541006 [3]中国科学院微生物研究所,北京100101

出　　处：《广西师范大学学报（自然科学版）》2018年第4期131-138,共8页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(31060120);广西研究生教育创新计划项目(XYCSZ2017070)

摘　　要：为了分离鉴定一株产天然蓝色素的细菌,本文以表面消毒法从牛大力Millettia specisoa Champ植物根中分离可产天然蓝色素的细菌,以通用引物27F/1492R对目标细菌的16SrDNA序列进行PCR扩增、序列对比分析、构建系统发育树,同时对目标细菌进行形态学观察,并采用GEN III MicroStation微生物自动鉴定系统对目标细菌进行Biolog鉴定。序列比对结果表明,目标细菌与Pseudomonas aeruginosa相近,相似率为99%;系统发育树显示,其与菌株Pseudomonas aeruginosa在同一条分支上;形态学鉴定为杆状革兰氏阴性;Biolog鉴定目标细菌为铜绿假单胞菌Pseudomonas aeruginosa。综合形态学、分子、Biolog鉴定结果,鉴定该细菌为铜绿假单胞菌,命名为2016NX1。In order to isolate and identify a bacterium producing natural blue pigment,surface sterilization method was applied to isolate producing natural blue pigment target strain from the root of Bovine Millettia specisoa Champ.16S rDNA sequence of the target strain was amplified by PCR with universal primer 27F/1492R,and the phylogenetic tree was constructed by comparing and analyzing the sequence.The morphology of the bacterium was observed at the same time.The target strain was identified by the GEN III MicroStation microorganism automatic identification system.The results of the sequence alignment showed that the similarity rate of target strain and Pseudomonas aeruginosa was 99%and the phylogenetic tree showed that the target strain was on the same branch of the P.aeruginosa.Morphological identification revealed that it was rod-shaped and Gram negative,and Biolog identification showed that it was P.aeruginosa.The target strain was identified as P.aeruginosa by the combined results of molecular,morphology and Biolog identification and named as 2016NX1.

关 键 词：天然蓝色素 16SrDNA Biolog鉴定 

分 类 号：Q939[生物学—微生物学]