聚ε-己内酯薄膜的脂肪酶促降解  

Study on Biodegradation of Polycaprolactone Films with Lipase

作　　者：马庆峰 李会举[2] 沈智奇 郭长友 苏婷婷[1] 王战勇[1] MA Qingfeng;LI Huiju;SHEN Zhiqi;GUO Changyou;SU Tingting;WANG Zhanyong(College of Chemistry,Chemical Engineering and Environmental Engineering,Liaoning Shihua University, Fushun 113001,China;School of Computer and Communication Engineering,Liaoning Shihua University, Fushun 113001,China;Dalian Research Institute of Petroleum&Petrochemicals,SINOPEC,Dalian 116045,China)

机构地区：[1]辽宁石油化工大学化学化工与环境学部,辽宁抚顺113001 [2]辽宁石油化工大学计算机与通信工程学院,辽宁抚顺113001 [3]中石化大连石油化工研究院,辽宁大连116045

出　　处：《中国塑料》2018年第10期117-122,共6页China Plastics

基　　金：国家自然科学基金资助项目(31570097)

摘　　要：利用脂肪酶对聚ε-己内酯(PCL)薄膜进行降解研究,并利用扫描电子显微镜、差示扫描量热仪和热重分析仪、X射线衍射、核磁共振以及质谱等分析方法对降解后的PCL薄膜进行了表征分析。结果表明,PCL薄膜(3cm×1cm×0.05cm)在45℃和30U/mL脂肪酶的作用下,经20h后失重率可达86.9%;随着降解时间的增加,PCL薄膜表面发生降解并形成孔洞,且孔洞随降解时间呈现增大趋势;PCL相对结晶度随降解时间的延长而逐渐降低;随降解时间的延长,PCL薄膜的热稳性呈下降趋势;PCL在酶促降解前后化学组成未发生变化。Poly(ε-caprolactone)(PCL)films were degraded by lipases and then were characterized by scanning electron microscopy(SEM),differential scanning calorimetry(DSC),thermogravimetric analysis(TGA),X-ray diffraction(XRD),nuclear magnetic resonance(NMR)and mass spectrometry(MS).The results indicated that the PCL films achieved a weight loss rate of 86.9 wt%when digested with 30 U/mL of lipase at 45℃for 20 h.SEM observation showed that there were lots of pores formed on the film surface with an increase of degradation time,and the size of pores tended to increase with degradation time.DSC and XRD analyses indicated that the relative crystallinity of PCL gradually decreased with an increase of degradation time.The thermal stability of PCL films was found to decrease with an increase of degradation time according to the TGA results.NMR analysis demonstrated that there was no change for PCL films in chemical composition before and after enzymatic degradation.

关 键 词：聚Ε-己内酯 脂肪酶 生物降解 

分 类 号：TQ321[化学工程—合成树脂塑料工业]