核黄素-紫外线A照射加速巩膜交联治疗豚鼠实验性近视  

Pulsed and continuous accelerated scleral cross-linking using riboflavin and ultraviolet A irradiation for the prevention of myopia progression in a guinea pig model

作　　者：徐海铭 刘辉 余洁 贺金晶 Xu Haiming;Liu Hui;Yu Jie;He Jinjing(Department of Ophthalmology,People's Hospital of Hangzhou Medical College,Zhejiang Provincial People's Hospital)

机构地区：[1]杭州医学院附属人民医院,浙江省人民医院眼科,杭州310014

出　　处：《中华实验眼科杂志》2018年第10期767-772,共6页Chinese Journal Of Experimental Ophthalmology

基　　金：浙江省医药卫生科技项目省部共建重点项目（2018273482）；浙江省医药卫生科技项目（2018233958）；浙江省中医药科技计划项目（2016ZA033）；浙江省公益技术应用研究计划实验动物研究项目（2015C37132）

摘　　要：目的评估口服核黄素联合脉冲和持续紫外线A（UVA）照射加速巩膜交联对实验性豚鼠进展性近视的治疗作用以及对巩膜生物力学和组织学改变作用。方法30只4周龄豚鼠按照随机数字表法分为空白对照组、非交联组、传统交联组、脉冲光加速交联组和持续光加速交联组，每组均为6只。3个组巩膜交联组豚鼠均连续服用核黄素和维生素C 3 d后，进行右眼近视眼建模和UVA照射。照射参数分别为传统交联组波长370 nm，功率为0.67 mW/cm 2，连续照射1 h；脉冲光加速交联组波长370 nm，功率为10 mW/cm 2，共照射8 min，脉冲模式为1 s开/1 s关；持续光加速交联组波长370 m，功率为10 mW/cm 2，连续照射4 min。非交联组仅接受右眼近视眼建模，但未服用核黄素及维生素C，也不接受UVA照射；空白对照组不接受任何处理。分别测量5个组基线及建模后的屈光度和眼轴长度。2周后颈椎脱臼法处死豚鼠，摘除右眼行巩膜生物力学和组织病理学检查。结果实验2周后，与空白对照组相比较，非交联组眼轴明显伸长，近视屈光度明显增加，巩膜最大载荷及最大应力显著下降，巩膜胶原纤维粗细不均匀，部分溶解，巩膜Collagen 1A及TIMP-2阳性染色减少，MMP2阳性染色增加，提示近视造模成功。传统交联组、脉冲光加速交联组和持续光加速交联组，交联组豚鼠眼球眼轴长度短于非交联组，和近视屈光度明显高于非交联组，差异均有统计学意义（均P<0.01）；3个交联组豚鼠眼球最大载荷值及最大应力值均高于非交联组[最大应力：（2.20±0.03)、（2.67±0.05）、（2.41±0.04）Mpa vs.(1.30±0.02）Mpa；最大载荷：（1.93±0.03）、（2.33±0.28）和（1.91±0.03）P vs.(1.54±0.06）P]，差异均有统计学意义（均P<0.01）；巩膜组织病理学检查显示脉冲光加速交联组和持续光加速交联组巩膜胶原纤维粗细均匀，更接近�Objective To evaluated the effect of oral administration of riboflavin combined with pulsed and continuous light accelerated scleral cross-linking on the histological and biomechanical properties of sclera in a guinea pig model to control the progression of myopia.Methods Thirty 4-week-old guinea pigs were divided into 5 groups,or the control group,non cross-linking group,conventional cross-linking group,pulse light cross-linking group and continuous light cross-linking group with 6 guinea pigs in each group.Three cross-linking groups were administered 0.1%riboflavin solution with vitamin C by gavage from 3 days before modeling to modeling process.The conventional cross-linking group underwent cross-linking with 1 hour of(ultraviolet A(UVA)exposure at 0.67 mW/cm 2,the pulse light cross-linking group received a pulsed-light accelerated crosslinking for 8 minuctes(1 second on/1 second off)of UVA exposure at 10 mW/cm 2,and the continuous light accelerated cross-linking group was crosslinked with continuous-light accelerated crosslinking at 10 mW/cm 2 for 4 minuctes.The same procedure was conducted on the non cross-linking group without UVA irradiation and 0.1%riboflavin solution before modeling and modeling process.No any intervene was carried out in the control group.Retinoscopy and the axial length measurement were performed before and after experiment.The animals were euthanized 2 weeks after experiment and then biomechanical and histopathological examinations of scleras were conducted.The use and care of the animals complied with Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission.Results Myopia models were established with an increased axial length and myopic diopter 2 weeks after myopic modeling process.Axial length in the non cross-linking group was longer than that of the control group at 2 weeks,with a siginificant difference between them(P<0.01).The myopic Diopter in the non cross-linking group was significantly increased in comprasion wi

关 键 词：核黄素 紫外线A 巩膜交联 近视 豚鼠 

分 类 号：R778.11[医药卫生—眼科]