杜仲内生青霉菌GZWMJZ-068次生代谢产物研究  

Secondary Metabolites of Endophytic Penicillium mallochii GZWMJZ-068 from Eucommia ulmoides

作　　者：龚倩玉 许言超 左明星 饶青 周彦伶[1,2] 朱伟明[1,4] 林昌虎 王立平 GONG Qian-yu;XU Yan-chao;ZUO Ming-xing;RAO Qing;ZHOU Yan-ling;ZHU Wei-ming;LIN Chang-hu;WANG Li-ping(State Key Laborataory of Functions and Applications of Medicinal Plants,Guizhou Medical University;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China;School of Medicine and Pharmacy,Ocean University of China, Qingdao 266003,China;Department of Pharmacology,Guizhou Medical University,Guiyang 550025,China)

机构地区：[1]省部共建药用植物功效与利用国家重点实验室贵州医科大学 [2]贵州医科大学药学院,贵阳550025 [3]贵州省中国科学院天然产物化学重点实验室,贵阳550014 [4]中国海洋大学医药学院,青岛266003

出　　处：《天然产物研究与开发》2018年第10期1721-1727,共7页Natural Product Research and Development

基　　金：贵州省"百人领军人才"计划;黔科合院士站([2015]4009号);黔科合体(Z字[2014]4007号)

摘　　要：为获得活性微生物代谢产物,从贵阳杜仲的叶片中分离得到内生真菌GZWMJZ-068,发酵产物用乙酸乙酯提取,经硅胶柱色谱、凝胶柱色谱和半制备高效液相色谱等进行分离纯化,得到15个单体化合物。运用核磁、质谱和比旋光度等方法鉴定了15个化合物的结构,即isochromophilones IV(1)、epi-isochromophilone III(2)、(2R,3S)-1-(4-羟基苯基)-2,3-丁二醇(3)、(2S,3S)-1-(4-羟基苯基)-2,3-丁二醇(4)、6-[1-羟基-(1S)-戊基]-4-甲氧基-(6S)-2H,5H-2-吡喃酮(5)、6-(1-羟戊基)-4-甲氧基-2-酮(6)、quinolactacins Al(7a)、quinolactacins A2(7b)、dihydrocitrinone(8)、dihydrocitrinin (9)、原儿茶酸(10)、儿茶酸(11)、5-甲基尿嘧啶(12)、尿嘧啶(13)、citrinal A(14)。对15个化合物进行了抑菌活性评价,化合物1和2对光滑念珠菌和金黄色葡萄球菌具有中等的抑制作用(MIC16-32μg/mL)。In order to investigate bioactive metabolites from endophytic fungi,a fungal GZWMJZ-068 was isolated and identified as Penicillium mallochii from the leaf of Eucommia ulmoides in Guiyang Guizhou,China.The extract of the fermentation was purified by column chromatography over silica gel,Sephadex LH-20 and semi-preparative HPLC.The structures were identified by means of NMR,MS and ORD.As a result,15 compounds,isochromophilones IV(1)、epi-isochromophilone III(2)、(2 R,3S)-1-(4-Hydroxyphenyl)-butane-2,3-diol(3)、(2 S,3S)-1-(4-Hydroxyphenyl)butane-2,3-diol(4)、6-[1-Hydroxy-(1 S)-pentyl]-4-methoxy-(6 S)-2H,5H-2-pyranone(5)、6-(1-hydroxypentyl)-4-methoxy-pyran-2-one(6)、quinolactacins Al(7a)、quinolactacins A2(7b)、dihydrocitrinone(8)、dihydrocitrinin(9)、protocatechuic acid(10)、pyrocatechuic acid(11)、5-Methyluracil(12)、uracil(13)、citrinal A(14).The antibacterial activities of 1-14 against 9 pathogenic microorganisms were tested by two-fold dilution method.Compounds 1 and 2 were active against Staphylococcus aureus and Candida glabrata with the minimum inhibitory concentration(MIC)values from 16μg/mL to 32μg/mL,respectively.

关 键 词：杜仲 内生真菌 代谢产物 抑菌活性 

分 类 号：R284.2[医药卫生—中药学]