急性胰腺炎患者尿液蛋白质组学分析  

Proteomics of urine in patients with acute pancreatitis

作　　者：杨妮[1] 白郑海[1] 邱晓娟 杨宁[1] 张丽[1] 武苗苗 裴红红[1] 张正良[1] YANG Ni;BAI Zhenghai;QIU Xiaojuan;YANG Ning;ZHANG Li;WU Miaomiao;PEI Honghong;ZHANG Zhengliang(The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710000,China)

机构地区：[1]西安交通大学第二附属医院,西安710000

出　　处：《山东医药》2018年第38期20-23,共4页Shandong Medical Journal

摘　　要：目的分析急性胰腺炎(AP)患者尿液中特异性生物标志物的蛋白质组学,寻找AP特异性生物标志物。方法收集15例AP患者(AP组)和15例健康查体者(对照组)的尿液标本,两组分别将每5例标本混合成1份(每组3份)。应用非标记质谱定量蛋白质组学技术检测尿液蛋白质谱,分析比较差异表达蛋白,通过生物信息学方法分析差异蛋白功能。选取筛选出的差异表达蛋白,采用Western blotting法检测其在AP组和对照组中的表达情况,验证蛋白质组学分析结果。结果两组共发现57个蛋白存在差异表达,AP组28个表达上调,29个表达下调;另有73个蛋白只在对照组中存在,9个蛋白只在AP组存在。差异表达蛋白主要参与对刺激的应答、免疫、代谢等过程的调控。生物信息学分析显示,糖蛋白CD59、免疫球蛋白重链IGHA2被富集在差异最显著的类别。Western blotting检测结果显示,AP组尿液中CD59蛋白表达较对照组升高,IGHA2蛋白表达较对照组降低。结论 AP患者与健康人的尿液蛋白质谱存在差异,糖蛋白CD59、免疫球蛋白重链IGHA2可能作为AP的特异性生物标志物。Objective To analyze the proteomics of specific biomarkers in acute pancreatitis(AP)patients and to find out the specific protein markers for AP.Methods We collected urine samples from 15 AP patients(AP group)and 15 healthy subjects(control group),and each of the 5 specimens was mixed into 1(3 in each group).We used non-labeled mass spectrometry quantitative proteomics to detect urinary protein profiles,analyzed the differentially expressed proteins,and analyzed the differential protein functions by bioinformatics methods.We selected the differentially expressed proteins and measured their expression in the AP group and the control group by Western blotting,and verified the proteomic analysis results.Results In this study,57 proteins were found to be differentially expressed in the two groups,in which 28 proteins were up-regulated and 29 proteins were down-regulated.Besides,another 73 proteins were found only in the control group,and 9 proteins were found only in the AP group.The differentially expressed proteins were mainly involved in the regulation of response to stimulation,immunity,and metabolism.Bioinformatics analysis found that glycoprotein CD59,immunoglobulin heavy chains were enriched in the most significant categories.The results of Western blotting showed that the expression of CD59 protein in the urine of the AP group was higher than that of the control group,and the expression of immunoglobulin heavy chain IGHA2 protein was lower than that of the control group.Conclusion There is a difference in the protein spectrum of urine between patients with AP and healthy persons,and glycoprotein CD59 and immunoglobulin heavy chain are expected to be specific biomarkers for patients with AP.

关 键 词：急性胰腺炎 非标记质谱定量蛋白质组学 差异表达蛋白 生物标志物 尿液 

分 类 号：R576[医药卫生—消化系统]