NaCl胁迫联合Ca^(2+)调控糙米发芽富集GABA的工艺优化  

Optimization of GABA Accumulation in Brown Rice under NaCl Stress and Calcium Regulation

作　　者：张亮 静恩岳 孙宇 孟宪梅 杨玉民 ZHANG Liang;JING En-yue;SUN Yu;MENG Xian-mei;YANG Yu-min(Grain College,Jilin Business and Technology College,Changchun 130507,Jilin,China)

机构地区：[1]吉林工商学院粮食学院,吉林长春130507

出　　处：《食品研究与开发》2018年第21期7-14,共8页Food Research and Development

基　　金：吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2015]第428号);粮油食品深加工吉林省高校重点实验室科研项目(吉高校重点实验室科合字[2014]第008号);吉林工商学院科学技术研究项目(院理科合字[L2015]第030号)

摘　　要：为优化NaCl胁迫联合Ca^(2+)调控下糙米发芽富集γ-氨基丁酸(γ-aminobutyric acid,GABA)的培养条件,通过单因素和Box-Behnken响应面试验考察NaCl浓度、Ca^(2+)浓度、发芽温度及发芽时间4个因素对GABA含量的影响,得出糙米发芽最佳工艺条件。结果表明,发芽糙米在NaCl胁迫联合Ca^(2+)处理下富集GABA的最佳培养条件为NaCl浓度7.50 mmol/L,Ca^(2+)浓度15.0 mmol/L,发芽温度29℃,发芽时间2.3 d,在此条件下发芽糙米中GABA含量为144.98mg/100g。研究结果为糙米健康食品的研究提供了一定的理论依据。The aim of this study was to optimize the culture condition forγ-aminobutyric acid(GABA)production in germinated brown rice.Effects of sodium chloride concentration,Ca^2+concentration,germination temperature and germination time were optimized by the combined used of single factor test and response surface methodology.Results demonstrated the optimum culture conditions of brown rice GABA accumulation under NaCl stress and calcium regulation were sodium chloride concentration 7.50 mmol/L,Ca^2+concentration 15.0 mmol/L,germination temperature 29℃and germination time 2.3 d.Under such conditions,the highest accumulation of GABA in brown rice was 144.98 mg/100 g.This finding will provide a guideline for further study of brown rice food.

关 键 词：糙米 发芽 Γ-氨基丁酸 响应面优化 NACL胁迫 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]