毛冬青叶中总黄酮提取工艺及其抗氧化性能研究  

Study on Extraction of Total Flavonoids from Leaves of Ilex pubescens and Its Antioxidation

作　　者：唐婷范[1] 邓起东 李晓慧 田玉红[1] TANG Ting-fan;DENG Qi-dong;LI Xiao-hui;TIAN Yu-hong(College of Biological and Chemical Engineering,Guangxi University of Science and Technoloy,Liuzhou 545006,Guangxi,China)

机构地区：[1]广西科技大学生物与化学工程学院,广西柳州545006

出　　处：《食品研究与开发》2018年第21期77-82,共6页Food Research and Development

基　　金：广西自然科学基金项目青年基金项目(2016GXNSFBA380073);广西高校中青年教师基础能力提升项目(KY2016YB250);广西高校重点实验室开放课题项目(2015TZYKF01);国家自然科学基金委员会资助项目地区科学基金项目(21662003);广西科技大学博士基金项目(校科博14Z06);广西科技大学"大学生创新创业训练计划"项目(201710594160)

摘　　要：以毛冬青叶为原料,进行毛冬青叶总黄酮提取工艺及其抗氧化性活性的研究,建立以芦丁为标准品,分光光度法测定毛冬青叶总黄酮含量的分析方法。结果显示:以60%乙醇的水为提取溶剂,浓度为60%、料液比为1∶60(g/mL)、50℃水浴5 h条件下提取1次,毛冬青叶总黄酮提取率为15.08%。其总黄酮化合物对羟基自由基(·OH)和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl radical,DPPH)自由基均有较强的清除效果,最大清除率分别为45.96%和83.33%。Extraction technology and antioxidant property of total flavonoids from leaves of Ilex pubescens was studied using Ilex pubescens as raw materials.The analytical method was established that total flavonoids content was determined by UV-spectrophotometric methods with rutin as standard.The results showed that the total flavonoids extraction rate reaches 15.08%under the optimized conditions of extraction at 50℃water bath for 5 hours with ethanol concentration of 60%as the solvent and the material/solvent ratio of 1∶60(g/mL).The clearing capacity of polyphenols for hydroxyl radical(·OH)and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical were very significant by hydroxyl radical and DPPH radical scavenging test,were 45.96%and 83.33%respectively.

关 键 词：毛冬青 总黄酮 提取工艺 抗氧化性 清除率 

分 类 号：TQ464[化学工程—制药化工]