甲状旁腺素相关肽蛋白核定位序列及C末端缺失对小鼠下颌磨牙发育的影响  

The influence of absence of parathyroid hormone-related peptide nuclear localization sequence and C-terminal on the development of mandibular molars in mice

作　　者：刘洪 颜成东 蒋尚飞 LIU Hong;YAN Chengdong;JIANG Shangfei(Department of Medical Technology,Jiangsu Vocational Collagen of Medicine, Yancheng,Jiangsu 224005,China)

机构地区：[1]江苏医药职业学院医学技术学院,江苏盐城224005

出　　处：《重庆医学》2018年第29期3748-3751,共4页Chongqing medicine

基　　金：盐城市医学科技发展计划项目(YK2016049)

摘　　要：目的研究甲状旁腺素相关肽(PTHrP)核定位序列和C-末端在小鼠下颌磨牙中的作用。方法选取野生型(WT)小鼠(WT组)和PTHrP Knock-in小鼠(PTHrP KI组)各10只,使用CT扫描三维重建、苏木精-伊红(HE)、免疫组织化学染色等方法分析PTHrP KI小鼠和WT小鼠的下颌第一磨牙的差异。结果 WT组小鼠的下颌第一磨牙明显长于PTHrP KI组小鼠,前期牙本质占总牙本质比例明显小于PTHrP KI组小鼠,差异均有统计学意义(P<0.01);WT组小鼠Ⅰ型胶原(Collagen-Ⅰ)、牙本质涎蛋白(DSP)和Ki-67阳性比例明显高于PTHrP KI组小鼠,而p27阳性比例低于PTHrP KI组小鼠,差异均有统计学意义(P<0.05)。结论 PTHrP核定位序列和C-末端缺失导致小鼠磨牙牙本质细胞外基质分泌减少,矿化障碍。Objective To study the effect of parathyroid hormone-related peptide(PTHrP)nuclear localization sequence and C-terminal on mice mandibular molars.Methods A total of 10 wild-type(WT)mice(WT group)and 10 PTHrP Knock-in mice(PTHrP KI group)were selected.The difference of the mandibular first molar between mice in the PTHrP KI group and mice in the WT group was detected by CT three-dimensional reconstruction,HE staining and immunohistochemical staining.Results The length of mandibular first molar of mice in the WT group was significantly longer than that of mice in the PTHrP KI group,while the percentage of predentin in total dentin was significantly smaller than that of mice in the PTHrP KI group,and the differences were statistically significant(P<0.01).In addition,the positive proportion of Collagen-Ⅰ,DSP and Ki-67 of mice in the WT group were significantly higher than those of mice in the PTHrP KI group,while the positive proportion of p27 was lower than that of mice in the PTHrP KI group,and the differences were statistically significant(P<0.05).Conclusion Absence of PTHrP nuclear localization sequence and C-terminal could lead to the reduction of secretion of extracellular matrix in molar dentin and disturbance of mineralization in mice.

关 键 词：甲状旁腺素相关肽 核定位序列 C末端 磨牙 发育 矿化 

分 类 号：R780.2[医药卫生—口腔医学]