肺腺癌EGFR、ALK突变与TTF-1表达的关系  

Correlation between EGFR,ALK mutation and TTF-1 expression in lung adenocarcinoma

作　　者：彭昆伟 何湘军[2] 罗漫君 尤长宣[1] PENG Kunwei;HE Xiangjun;LUO Manjun;YOU Changxuan(Department of Oncology, Southern Medical University,Guangzhou,Guangdong 510515,China;Hui Qiao Medical Center,Nan Fang Hospital, Southern Medical University,Guangzhou,Guangdong 510515,China)

机构地区：[1]南方医科大学南方医院肿瘤科,广州510515 [2]南方医科大学南方医院惠侨医疗中心,广州510515

出　　处：《重庆医学》2018年第29期3763-3765,3769,共4页Chongqing medicine

基　　金：广东省科技计划项目(2017B090901067)

摘　　要：目的分析肺腺癌表皮生长因子受体(EGFR)、间变性淋巴瘤激酶(ALK)基因突变与甲状腺转录因子-1(TTF-1)表达之间的关系。方法收集南方医科大学南方医院2016年1月至2017年5月收治的肺腺癌患者242例,采用免疫组织化学法检测TTF-1表达、ALK突变,采用扩增阻滞突变系统法检测EGFR突变情况,分析EGFR、ALK突变与TTF-1表达的关系。结果 TTF-1阳性患者中,检测出79例EGFR突变,23例ALK突变。TTF-1阴性患者中,检测出2例EGFR突变,未检出ALK突变者。TTF-1阳性患者的EGFR基因突变率高于阴性患者,差异有统计学意义(46.74%vs.8.00%,P<0.05);TTF-1阳性患者的ALK基因突变率高于阴性患者,差异无统计学意义(11.68%vs.0,P>0.05)。TTF-1预测EGFR、ALK突变的阴性预测值分别为92.00%、100.00%。结论在肺腺癌中,EGFR、ALK突变少见于TTF-1阴性患者。Objective To investigate the correlations between epidermal growth factor receptor(EGFR),anaplastic lymphoma kinase(ALK)mutation and thyroid transcription factor-1(TTF-1)expression in lung adenocarcinoma.Methods A total of 242 cases of patients with lung adenocarcinoma were collected from Nan Fang Hospital,Southern Medical University from January 2016 to May 2017.Immunohistochemistry was used to detect the expression of TTF-1 and the mutation of ALK,amplification refractory mutation system was used to detect the EGFR mutation.The relationships between EGFR,ALK mutation and TTF-1 expression were analysed.Results Among the TTF-1 positive patients,79 cases of EGFR mutation and 23 cases of ALK mutation were found.Among the TTF-1 negative patients,2 cases of EGFR mutation was observed,and none of ALK mutation was found.The mutation rate of EGFR in the TTF-1 positive cases was higher than that in the TTF-1 negative cases,the difference was statistically significant(46.74%vs.8.00%,P<0.05);the mutation rate of ALK in the TTF-1 positive cases was also higher than that in the TTF-1 negative cases,but no statistically significant difference was found(11.68%vs.0,P>0.05).The negative predictive value of TTF-1 for predicting EGFR and ALK mutation was 92.00%and 100.00%,respectively.Conclusion In lung adenocarcinoma,EGFR and ALK mutation are exceptionally rare in TTF-1 negative patients.

关 键 词：肺肿瘤 腺癌 受体 表皮生长因子 间变性淋巴瘤激酶 甲状腺转录因子-1 

分 类 号：R734.2[医药卫生—肿瘤]