铁皮石斛多糖对慢性萎缩性胃炎大鼠的作用及其分子机制研究  

The effect and ’molecular mechanism of Dendrobium candidum polysaccharides on chronic atrophic gastritis in rats

作　　者：欧阳一鸣[1] 凌平[1] 李临海[1] 龙亚新[1] 朱宇[1] 王文荣[1] OUYANG Yiming;LING Ping;LI Linhai;LONG Yaxin;ZHU Yu;WANG Wenrong(First People’s Hospital of Yunnan Province,Kunming 650000,China)

机构地区：[1]云南省第一人民医院,昆明650000

出　　处：《中国比较医学杂志》2018年第10期67-72,78,共7页Chinese Journal of Comparative Medicine

摘　　要：目的探讨铁皮石斛多糖对亚硝基胍(N-methyl-N′-nitro-N-nitrosoguanidine,MNNG)诱发的大鼠慢性萎缩性胃炎(chronic atrophic gastritis,CAG)的作用及其分子机制。方法 120只大鼠随机分为正常组,模型组,养胃舒组,铁皮石斛多糖高、中、低剂量组,每组20只。模型组,养胃舒组,铁皮石斛多糖高、中、低剂量组给予167μg/mL MNNG饮用液,正常组自由饮用蒸馏水,共造模8周。造模成功后,铁皮石斛多糖高剂量组每日灌胃给予铁皮石斛多糖提取物生药量1.34 g/kg;中剂量组0.67 g/kg;低剂量组0.335 g/kg。养胃舒组每日灌胃给予养胃舒颗粒4.0 g/kg。模型组给予生理盐水灌胃,正常组不予干预。给药12周后,取出胃组织,进行病理组织学、胃泌素(gastrin,Gas)、白介素-6(IL-6)、增殖细胞核抗原(proliferatingCellNuclearAntigen,PCNA)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-αmRNA)、Janus激酶2(JAK2)、激活因子-3/酪氨酸磷酸化3(p-STAT3/STAT3)的检测。结果治疗第12周后,正常组,铁皮石斛多糖高、中、低剂量组、养胃舒组的体重均高于模型组(P<0.05)。铁皮石斛多糖高、中剂量组体重高于养胃舒组(P<0.05);②模型组炎症等级高于正常组(P<0.01),而腺体数量、腺体厚度、主细胞数量、壁细胞数量低于模型组(P<0.05)。铁皮石斛多糖高、中、低剂量组,养胃舒组的炎症等级、腺体数量、腺体厚度、主细胞数量、壁细胞数量较模型组均有恢复(P<0.05)。与养胃舒组比较,铁皮石斛多糖高、中剂量组的主细胞数量增长(P<0.05);铁皮石斛多糖高剂量组的壁细胞数量增长(P<0.05);③正常组,铁皮石斛多糖高、中、低剂量组,养胃舒组的血清GAS含量高于模型组(P<0.05)。正常组,铁皮石斛多糖高剂量组的血清IL-6含量低于模型组(P<0.05);④正常组,铁皮石斛多糖高、中剂量组的PCNA、TNF-αmRNA低于模型组(P<0.05);⑤正常组,铁皮石斛多糖高、中剂量组JAK2、p-STAT3/STAT3�Objective To investigate the effect and molecular mechanism of Dendrobium candidum polysaccharides on chronic atrophic gastritis(CAG)induced by N-methyl-N′-nitro-N-nitrosoguanidine(MNNG)in rats.Methods To establish the CAG model,Wistar rats were first provided free access to MNNG drinking solution containing 167 g/mL distilled water for 8 weeks.Then,the high-dose Dendrobium candidum polysaccharide group was given 1.34 g/kg of raw drug per day by gavage,the medium-dose group was given 0.67 g/kg,and the low-dose group was given 0.335 g/kg.Weiyangshu granules at 4.0 g/kg were administered to the groups every day.Normal saline was administered to a model group,in which no intervention was performed.Twelve weeks after the treatment,the stomach tissue was removed,and subjected to analyses of the histology and levels of stomach gastrin release,interleukin-6,PCNA,tumor necrosis factor alpha,Janus kinase 2,and activating factor-3/tyrosine phosphorylation-3.Results 1)After 12 weeks of treatment,the body weight of the model group was significantly lower than that of the normal group(P<0.01)..The weights of the three groups with different dosages of Dendrobium polysaccharide and the Yangweishu group were significantly higher than that of the model group(P<0.05).The body weight of the high-dose Dendrobium candidum polysaccharide group was significantly higher than that of the Yangweishu group(P<0.05).(2)The grade of inflammation in the model group was significantly higher than that in the normal group(P<0.01).The numbers and thicknesses of chief cells and parietal cells in the model group were significantly lower than those in the normal group(P<0.05),while the number of glands in the normal group and the thickness of the rats were thick and thick chief cells and parietal cells were significantly higher than those in the model group(P<0.05).In the three dose groups of Dendrobium candidum polysaccharide,the grade of inflammation of gastric tissue,number of glands,gland thickness,chief cell,and parietal cells in the Yangweish

关 键 词：铁皮石斛多糖 养胃舒 慢性萎缩性胃炎 机制 

分 类 号：R-33[医药卫生]