幼羊颅骨缺损模型中颅骨组织Dlx2基因的表达及意义分析  

Expression and significance of Dlx2 gene in skull defect model of young sheep

作　　者：毕玉杰 周昊 崔阳 邵国[2,3,4] 张春阳[1] BI Yujie;ZHOU Hao;CUI Yang;SHAO Guo;ZHANG Chunyang(Department of Neurosurgery in The First Affiliated Hospital of Baotou Medical College of Inner Mongolia University of Science and Technology,Baotou 014010,China;Biomedicine Research Center, Baotou 014040;Department of Biochemistry and Molecular Biology,Baotou Medical College of Inner Mongolia University of Science and Technology,Baotou 014040;Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine,Xuanwu Hospital,Capital Medical University,Beijing 100053)

机构地区：[1]内蒙古科技大学包头医学院第一附属医院神经外科,包头014010 [2]内蒙古包头医学院生物医学研究中心,包头014040 [3]内蒙古科技大学包头医学院生物化学及分子生物学教研室,包头014040 [4]首都医科大学宣武医院低氧适应转化医学北京市重点实验室,北京100053

出　　处：《中国比较医学杂志》2018年第10期73-78,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(81360164;81660214);内蒙古自然科学基金(2015MS0866;2015MS0859);包头市医药卫生基金(wsjj2016042;2015S2004-5-10);包头医学院科学基金(BYJJ-YF201697;BYJJ-QM2016119)

摘　　要：目的检测Dlx2基因在小尾寒羊颅骨缺损模型颅骨组织中的表达,探讨其对颅骨缺损的意义。方法选取10只1月龄小尾寒羊,建立颅骨缺损模型,对缺损部位的新生颅骨组织及邻近缺损部位的原生颅骨组织进行取材,应用Real-time PCR和Western blot技术检测Dlx2的mRNA和蛋白表达水平。结果与邻近缺损部位的原生颅骨组织比较,缺损部位的新生颅骨组织Dlx2 mRNA和蛋白表达水平显著升高(P<0.05)。结论Dlx2基因表达增加可能对颅骨缺损修复有重要意义。Objective To determine the expression of the Dlx2 gene in skull tissue of the small-tailed Han sheep skull defect model,and to investigate its significance in this skull defect.Methods Ten small-tailed Han sheep aged one month old were selected to establish the skull defect model.Newborn skull tissue in the defect area and native skull tissue adjacent to the defect area were collected.Dlx2 expression at the mRNA and protein levels was determined by real-time PCR and western blotting.Results Compared with the levels in native skull tissue adjacent to the defect area,the Dlx2 mRNA and protein expression of newborn skull tissue in the defect area was significantly increased(P<0.05).Conclusions Increased Dlx2 gene expression may play an important role in the repair of skull defects.

关 键 词：颅骨 修复 PCR Dlx2基因 

分 类 号：R-33[医药卫生]