TLR4的表达在急性缺血性脑血管病中作用的研究  

The research about expression of TLR4 in acute ischemic cerebrovascular disease

作　　者：王辰[1] 秦丽微[2] 秦丽红[3] 关雪莲[1] 石磊[1] 王秀萍[1] 侯丽淳[1] WANG Chen;QIN Liwei;QIN Lihong(The first Affiliated Hospital of Jiamusi University,Jamusi 154002,China)

机构地区：[1]佳木斯大学附属第一医院神经二科,黑龙江佳木斯154002 [2]佳木斯大学附属第一医院物理诊断科,黑龙江佳木斯154002 [3]佳木斯大学附属第一医院神经一科,黑龙江佳木斯154002

出　　处：《中风与神经疾病杂志》2018年第10期916-918,共3页Journal of Apoplexy and Nervous Diseases

基　　金：佳木斯大学基础研究类面上项目(No.JMSUJCMS2016-072)

摘　　要：目的观察急性缺血性脑血管病患者外周血单核细胞TLR4的表达及其与早期脑梗死中典型炎症因子TNF-α、IL-6的相关性,旨在探讨TLR4在急性脑梗死中发挥的炎症损伤的作用。方法研究对象的选取分为3组:(1)发病3 d的急性脑梗死组;(2)无急性脑梗死的脑动脉粥样硬化组;(3)健康人群组。组间比较3组外周血单核细胞TLR4所表达的TLR4mRNA、外周血中TNF-α、IL-6浓度。急性脑梗死外周血单核细胞TLR4 mRNA的表达及其与TNF-α、IL-6浓度相关性。结果急性脑梗死组TLR4 mRNA的表达和血清TNF-α、IL-6浓度均显著高于动脉粥样硬化组和健康人群组(P <0. 01)。急性脑梗死患者TLR4 mRNA与血清炎性因子TNF-α、IL-6浓度均呈正相关(P <0. 01)。结论 TLR4在急性脑梗死发病机制中炎性损伤的过程有着重要作用,通过上调TLR4表达,推测可能是促使炎性因子TNF-α、IL-6等浓度增多,来介导脑缺血的炎性损伤。Objective In order to study the role of TLR4 in acute cerebral infarction,we observed the correlation of the expression of peripheral blood mononuclear cells TLR4 mRNA and TNF-α,IL-6 in the inflammatory injury of acute cerebral infarction.Methods The selection of the research object is divided into three groups:(1)Patients with acute cerebral infarction group at the third day;(2)Atherosclerosis group;(3)Healthy people group.Groups were compared with concentration of TNF-α,IL-6,and the correlation of TLR4 mRNA expression in three sets of peripheral blood mononuclear cells.Results The concentration of TLR4 mRNA and serum TNF-α,IL-6,of cerebral infarction group were significantly higher than that of atherosclerosis group and healthy group(P<0.01).The expression of TLR4 mRNA was correlated positively with the concentration of TNF-α,IL-6(P<0.01).Conclusion TLR4 plays an important role in the process of inflammatory injury of the pathogenesis of cerebral infarction,which prompted inflammatory factor TNF-α,IL-6 concentration increased to result in inflammatory injury of cerebral ischemia.

关 键 词：急性脑梗死 TLR4 MRNA TNF-Α IL-6 

分 类 号：R743.3[医药卫生—神经病学与精神病学]