AEG-1调控eIF4E表达促进胃癌细胞迁移的作用机制  

AEG-1 induces metastasis of gastric cancer by regulation of eIF4E expression

作　　者：徐可心 吴丹丹[1] 吴圣洁 王雪融[1] XU Kexin;WU Dandan;WU Shengjie;WANG Xuerong(Department of Pharmacology,Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学基础医学院药理学系,江苏南京210029

出　　处：《东南大学学报（医学版）》2018年第5期765-770,共6页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(81473241;81172004;81102458;30873099)

摘　　要：目的:探讨AEG-1调控e IF4E表达促进胃癌细胞迁移的作用机制。方法:在SGC7901胃癌细胞株上用质粒转染或siRNA转染的方法过表达或沉默AEG-1的表达,用Western blotting法检测eIF4E及上皮-间质样转化(EMT)的标志物表达的变化,用transwell实验检测胃癌细胞的迁移。结果:过表达AEG-1诱导SGC7901细胞发生EMT,同时上调eIF4E的表达;沉默AEG-1的表达抑制细胞发生EMT,同时下调eIF4E的表达;沉默AEG-1的表达抑制CXCL12诱导的细胞迁移,同时下调eIF4E的表达;过表达eIF4E部分逆转由于沉默AEG-1的表达引起的胃癌细胞迁移的抑制作用。结论:AEG-1通过上调eIF4E表达促进胃癌细胞的迁移。Objective:To explore the effect and mechanism of AEG-1 in the metastasis of gastric cancer via the regulation of eIF4E.Methods:Overexpress or silence the expression of AEG-1 by plasmid transfection or siRNA transfection on SGC7901 gastric cancer cell lines.Western blotting analysis was used to detect the protein level of eIF4E and epithelial-mesenchymal transition(EMT).Cell migration was detected by transwell assay.Results:Overexpression of AEG-1 induced EMT as well as upregulation of eIF4E expression.Silence of AEG-1 expression inhibited EMT in parallel with downregulation of eIF4E expression.Silence of AEG-1 expression inhibited CXCL12-induced cell migration in parallel with downregulation of eIF4E expression.Overexpression of eIF4E promoted cell migration in AEG-1-silencing induced inhibition of cell migration.Conclusion:AEG-1 induces migration of gastric cancer cell via upregulation of eIF4E expression.

关 键 词：胃癌 星形胶质细胞上调基因-1 真核翻译起始因子4E 上皮-间质样转化 

分 类 号：R735.2[医药卫生—肿瘤] Q279[医药卫生—临床医学]