益气固本胶囊调控哮喘小鼠核因子κB信号通路肿瘤坏死因子α和白细胞介素1β含量的实验研究  

Experimental study on the effect of Yiqi Guben capsule in regulating the content of TNF-α、IL-1β in NF-κB signaling pathway of asthma mice

作　　者：孔一卜 孙丽平[2] 刘璐[1] KONG Yibu;SUN Liping;LIU Lu(Changchun University of Traditional Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学,长春130117 [2]长春中医药大学附属医院儿童诊疗中心,长春130021

出　　处：《中国中西医结合儿科学》2018年第5期409-412,共4页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金面上项目(81473729);吉林省科技发展计划项目(20150101209JC)

摘　　要：目的观察益气固本胶囊对哮喘小鼠血清、肺泡灌洗液中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)含量的影响,探讨益气固本胶囊抑制哮喘气道重塑的作用机制。方法将60只BALB/c小鼠随机分为正常对照组(10只)、模型组(14只)、益气固本胶囊组(12只)、地塞米松片组(12只)、童康片组(12只),将益气固本胶囊(55g/L)、地塞米松片(0.005g/L)、童康片(44g/L)给予各药物组灌胃,正常对照组、模型组给予等容积的纯净水灌胃,每日1次,连续30d。根据文献采用卵蛋白(OVA)致敏建立小鼠哮喘模型。给药后的第21天激发哮喘模型,每日激发1次,连续10d,激发前1h药物组给予受试药。用酶联免疫吸附试验(ELISA)法测定给药30d后小鼠血清和肺泡灌洗液中TNF-α、IL-1β的含量。结果模型组小鼠血清中TNF-α与正常对照组比较含量升高,差异有统计学意义(P<0.01)。益气固本胶囊组、童康片组、地塞米松组小鼠血清中TNF-α与模型组比较含量降低,差异有统计学意义(P<0.05),IL-1β含量无明显差异(P>0.05)。模型组肺泡灌洗液中TNF-α、IL-1β与正常对照组比较含量升高,差异有统计学意义(P<0.01)。益气固本胶囊组、童康片组、地塞米松组小鼠肺泡灌洗液中TNF-α、IL-1β与模型组比较含量降低,差异有统计学意义(P<0.05)。结论益气固本胶囊可有效降低哮喘小鼠血清中TNF-α及肺泡灌洗液中TNF-α、IL-1β的含量,推测其可能是通过降低小鼠体内TNF-α、IL-1β的含量抑制NF-κB信号通路的活性从而发挥治疗哮喘的作用。Objective To observe the effect of Yiqi Guben capsule on the levels of TNF-α、IL-1βin serum and alveolar lavage fluid of asthmatic mice,and to explore the mechanism of Yiqi Guben capsule in inhibiting airway remodeling in asthma.Methods Sixty BALB/c mice were randomly divided into normal control group(n=10),model group(n=14),Yiqi Guben capsules group(n=12),dexamethasone tablets group(n=12)and Tongkang tablets group(n=12).Yiqi Guben capsules(55 g/L),dexamethasone tablets(0.005 g/L)and Tongkang tablets(44 g/L)were given by intragastric administration to each drug group,and normal control group and model group were given equal volume of pure water by intragastric administration,once a day for 30 consecutive days.Asthma mice models were established by sensitization with ovalbumin(OVA)according to the literature.Asthma models were challenged once a day for 10 consecutive days beginning on the 21 st day after administration.The drug groups were given the tested drug 1 hour before the provocation.The content of TNF-αand IL-1βin serum and alveolar lavage fluid were determined by enzyme-linked immunosorbent assay(ELISA)after 30 days.Results Compared with normal control group,Serum levels of TNF-αin model group statistically were significantly increased(P<0.01).Compare with asthma model group,serum levels of TNF-αin Yiqi Guben capsule group and Tongkang tablet group and dexamethasone tablets group were decreased significantly(P<0.05),serum levels of IL-1βwas no statistical significance(P>0.05).Compare with normal control group,bronchoalveolar lavage fluid levels of TNF-αand IL-1βin model group were significantly increased(P<0.01).Compare with asthma model group,bronchoalveolar lavage fluid levels of TNF-αand IL-1βin Yiqi Guben capsule group and Tongkang tablet group and dexamethasone tablets were decreased significantly(P<0.05).Conclusion Yiqi Guben capsule can effectively reduce the levels of TNF-αin serum and TNF-α,IL-1βin bronchoalveolar lavage fluid of asthmatic mice.It is suggested that it may treat

关 键 词：哮喘 益气固本胶囊 NF-κB 气道重塑 小鼠 

分 类 号：R36[医药卫生—病理学]