光照等条件对G03菌株繁殖及其ZwA合成的影响  

Light intensity effect and other factors on metabolism growth and ZwA synthesis of G03

作　　者：陈圣怡 李霞[1,2] 刘建国[1,2] 温心遥 程桂阳 郝再彬[1,2] CHEN Sheng-yi;LI Xia;LIU Jian-guo;WEN Xin-yao;CHENG Gui-yang;HAO Zai-bin(Guangxi College and University Key Laboratory of Food Safety and Detection,Guilin University of Technology,Guilin 541004,China;College of Chemistry and Bioengineering,Guilin University of Technology,Guilin 541004,China)

机构地区：[1]桂林理工大学广西高校食品安全与检测重点实验室,广西桂林541004 [2]桂林理工大学化学与生物工程学院,广西桂林541004

出　　处：《桂林理工大学学报》2018年第3期537-541,共5页Journal of Guilin University of Technology

基　　金：植物病虫害生物学国家重点实验室项目(SKLOF201404);广西自然科学基金项目(2014GXNSFBA118134)

摘　　要：以苏云金芽胞杆菌(Bacillus thuringiensis,Bt) G03菌株为对象,研究光照、碳源、氮源、无机盐、培养基初始pH和培养温度等条件对该菌株繁殖及其代谢产物杀虫晶体蛋白和Zwittermicin A(ZwA)活性的影响。结果表明,G03菌株发酵培养基的最适碳源为葡萄糖,最适氮源为酵母粉,最适无机盐为氯化钠,最适培养温度为30℃,最适的初始pH为7～7. 5; ZwA的最适合成条件与之相同,但是其最适初始pH为8. 0;菌株在4 000lux下发酵40 h时繁殖量最大;杀虫晶体蛋白积累量在4 000 lux下发酵55 h时最大;黑暗条件下ZwA的积累量较2 000 lux光强下的大。In this paper the effect of light intensity,carbon source,nitrogen source,inorganic salt,temperature and initial pH on the metabolism growth of G03 is discussed by detecting the growth rate of G03 and the biological activity of metabolite Zwittermicin A.The accumulation of insecticidal crystal protein and ZwA under different light intensity is detected traceably.Consequently,the optimal carbon source of G03 for fermentation is glucose,the optimal nitrogen source is yeast extract,the optimal inorganic salt is NaCl,the optimal temperature is 30℃and the optimal initial pH is 7~7.5.The optimal condition of ZwA is the same with fermentation,but the optimal initial pH is 8.0.The bacterial density has a maximum accumulation at 40 h under 4 000 lux light intensity.And the insecticidal crystal proteins have a maximum accumulation at 55 h under 4 000 lux light intensity.The accumulation of ZwA under dark condition is more than 2 000 lux.

关 键 词：苏云金芽胞杆菌(Bt) G03菌株 光照强度 杀虫晶体蛋白 ZwittermicinA(ZwA) 

分 类 号：TQ920[轻工技术与工程—发酵工程]