氟烷基因在皮特兰猪群体中的分子标记辅助选择研究  

Marker-assisted selection of RYR1 gene in Pietrain pig population

作　　者：杨明[1] 温淑贤[1] 王青来[1] 刘珍云 曾海玉[1] 吴珍芳[1,2] 蔡更元[1,2] YANG Ming;WEN Shuxian;WANG Qinglai;LIU Zhenyun;ZENG Haiyu;WU Zhenfang;CAI Gengyuan(Guangdong Wens Foodstuff Co.,Ltd./National Engineering Research Center for Breeding Swine Industry,Xinxing 527439,China;College of Animal Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]广东温氏食品集团股份有限公司/国家生猪种业工程技术研究中心,广东新兴527439 [2]华南农业大学动物科学学院,广东广州510642

出　　处：《华南农业大学学报》2018年第6期1-4,共4页Journal of South China Agricultural University

基　　金：广东省应用型科技研发专项资金项目(2015B020231010);广东省"扬帆计划"引进创新创业团队项目(2016YT03H062)

摘　　要：【目的】探索氟烷基因(RYR1)在皮特兰猪群体中的辅助选择育种方法,以提高猪群的猪肉质量。【方法】利用PCR-RFLP的方法检测509头皮特兰猪中RYR1基因的等位基因频率,利用回归模型分析基因型与选育性状之间的相关性,采用Bootstrap方法分析等位基因频率与选育性状的相关性。【结果】在皮特兰猪群体中RYR1基因优势等位基因(N)频率占81.73%。NN基因型个体在日增质量方面表现明显优势,在背膘EBV、父系指数及母系指数中Nn基因型个体表现较好,nn基因型个体在肢蹄方面表现好于其他2种基因型。n等位基因频率达19%时,选育性状表现平衡;大于19%时,则瘦肉率较高,背膘较薄,体型较好;小于19%时,则生长速度较快。【结论】若皮特兰猪选育方向更注重瘦肉率、背膘厚、体型外貌等,可不优化RYR1基因;若是更注重生长速度、饲养抗应激、屠宰后肉质等方面的性状改良,则建议全面纯化皮特兰猪群体至RYR1基因优势等位基因纯合。【Objective】To develop marker-assisted selection(MAS)technology of RYR1 gene in breeding of Pietrain pig populations,and improve pork quality in pig populations.【Method】PCR-RFLP assay was used to detect the allele frequency of RYR1 gene in 509 Pietrain pigs.The correlation between genotype and phenotype was analyzed using regression model.The correlation between allele frequency and phenotype was analyzed using the Bootstrap method.【Result】The predominant allele(N)frequency of RYR1 gene was 81.73%in the Pietrain pig population.The pigs with NN genotype had better performance in daily gain,the pigs with Nn genotype were better in backfat EBV,maternal and paternal indexes,and the pigs with nn genotype were better in limb trait.Breeding performance was in balance when n allele frequency was 19%.When n allele frequency was above 19%,the pigs had higher lean meat content,thinner backfat,and better body shape.When n allele frequency was below 19%,the pigs had higher growth rate.【Conclusion】When body shape,lean meat content,back fat thickness were more important goals for Pietrain pig breeding,it is suggested to increase n allele frequency in the population.When growth rate,anti-stress,and meat quality after slaughter are more important goals for breeding,it is suggested to purify the predominant allele(N)of RYR1 gene in the population.

关 键 词：氟烷基因 皮特兰猪 分子标记辅助选择 基因型 等位基因频率 

分 类 号：S813.3[农业科学—畜牧学]